Skip to navigationSkip to contentSkip to footer
Need Help?770-729-2992
MY ACCOUNT
CART
RayBiotech
RayBiotech
  • Products
    • Multiplex Assays
    • ELISA Kits
    • Proteins
    • Antibodies
    • Flow Cytometry
    • Assay Kits
    • Molecular Biology
    • Other Products
    • Human Cytokine Array C5
    • Human IL-6 ELISA
    • Human Inflammation Array Q3
    • Mouse Cytokine Array C3
    • Recombinant SARS-CoV-2 Spike Protein, S1 Subunit
    • Human Phosphorylation Pathway Profiling Array C55
    • Antibody Arrays
    • Proteome Profiling Arrays
    • Cytometric Bead Arrays
    • Epitope Mapping Peptide Arrays
    • Protein Arrays
    • PTM Multiplex Assays
    • NexaTag® Ultrasensitive Multiplex ELISA
    • Sandwich ELISA
    • Competitive ELISA
    • PTM ELISA
    • NexaTag® Ultrasensitive qIPCR ELISA
    • Indirect ELISA
    • Bridging ELISA
    • Cell-Based ELISA
    • Recombinant Proteins
    • GMP Proteins
    • Native Proteins
    • Peptides
    • Primary Antibodies
    • Secondary Antibodies
    • Flow Cytometry Antibodies
    • Isotype Controls
    • Antibody Pairs
    • Flow Cytometry Antibodies
    • Flow Cytometry Reagents
    • Flow Cytometry Assays
    • Cytometric Bead Arrays
    • Singleplex Bead Assays
    • Fluorescent Beads
    • Quantum Dots
    • Metabolism Assays
    • Ligand Binding Assays
    • Drug Antibody (DA) & ADA Assays
    • Transcription Factor Activity Assays
    • PCR Assays
    • Nucleic Acid Assays
    • Epigenetics
    • Mitochondrial Assays
    • Reagents
    • Cell Culture
    • Biospecimen Samples
    • Sample Collection & Preparation
    • Lysates
    • Magnetic Beads
    • Lab Equipment and Supplies
    • Biological Buffers

    Featured Products

    Human Cytokine Array C5Human IL-6 ELISAHuman Inflammation Array Q3Mouse Cytokine Array C3Recombinant SARS-CoV-2 Spike Protein, S1 SubunitHuman Phosphorylation Pathway Profiling Array C55
  • Services
    • Multiplex Assay Services
    • ELISA Services
    • Protein Services
    • Antibody Services
    • CRO Services
    • Flow Cytometry Services
    • SIMOA – Single Molecule Array
    • Other Services
    • Proteomic Services
    • Custom Arrays
    • Array Scanning and Analysis
    • Custom Protein Production
    • GMP Protein Production
    • IHC Controls
    • Stable Cell Line Development
    • Molecular Biology Services
    • Antibody Production
    • Recombinant Antibody Expression
    • Bulk Antibody Production
    • Antibody Conjugation Services
    • Cell Biology Services
    • Epitope Mapping Services
    • Biomarker Discovery
    • Cell Biology Services
    • Antibody Drug Development & Characterization
    • Pharmacokinetic and Pharmacodynamic Analysis
    • Assay Development Services
    • Molecular Biology Services
    • Multiomics Services
    • Diagnostic Assay Development
    • GMP Protein Production
    • Biostatistics and Bioinformatics
    • Bulk Antibody Production
    • QC Testing Services
    • Auto-Western Blot Service
    • COVID-19 Pseudovirus Service
    • Biospecimen Samples & Sample Services
    • RNA & DNA Methylation Detection Services
  • Research Areas
    • Cell Signaling Pathways
    • Areas of Interest
    • Akt Signaling Pathway
    • AMPK Signaling pathway
    • ErbB Signaling Pathway
    • Hedgehog Signaling Pathway
    • HIF1-alpha Signaling Pathway
    • IGFR1 Signaling Pathway
    • JAK-STAT Signaling Pathway
    • MAPK Signaling Pathway
    • mTOR Signaling Pathway
    • NF-kappaB Signaling Pathway
    • Notch Signaling Pathway
    • p53 Signaling Pathway
    • PKC Pathway
    • TGF-beta Signaling Pathway
    • Wnt/beta-Catenin Pathway
    • Antibody Drug Development
    • Autoimmunity & Inflammation
    • Cancer
    • Cardiovascular Disease
    • Infectious Disease & Vaccines
    • Neuroscience
    • Obesity
    • Post Translational Modifications
    • RNA & DNA Modifications
  • Resources
    • Array Picker Tool
    • Sample Shipment Instructions
    • Sample Preparation Tips
    • Publications / Citations
    • Promotions
    • Resource Library
    • Learning Center
    • Manual Protocols
    • Array Analysis Tools
  • Contact Us
    • Distributors & Service Providers
  • About Us
    • Quality Systems
    • Business Partnerships
    • Careers
    • News & Events
Custom ELISA development
  • Home
  • ELISA Services
  • Custom ELISA Development

Custom ELISA Development

Can’t find an ELISA for your exact target, modification, or sample type? We’ll build one.

Founded in 2001, RayBiotech designs, develops, and manufactures ELISA kits in-house in Peachtree Corners, Georgia. Our PhD scientists take your assay from feasibility through validation.

Request a QuoteELISA Testing Services

What We Can Customize

  • Post-translational modifications: Measure the modified form of your protein, alone or alongside total protein
  • Sample types: Validate an existing assay in your matrix, using samples you provide
  • Higher sensitivity: If standard ELISA can’t reach your levels, we’ll recommend a more sensitive platform such as Simoa® or NexaTag® immuno-PCR
  • Bulk orders: Bulk discounts and long-term supply agreements for large studies
  • Species: Other species may be possible when suitable antibodies and reagents exist. Ask us about yours

PTM-Specific ELISAs

Many proteins change function once they’re modified, and a total-protein ELISA can’t tell you which form is present. We develop ELISAs that detect specific modifications, including:

Phosphorylation · Glycosylation · O-GlcNAcylation · Glycation · Acetylation · Acylation · Palmitoylation · Lactylation · Succinylation · Methylation · Citrullination · Ubiquitination · SUMOylation · S-Nitrosylation · Nitration · Oxidation

Every PTM-specific assay is tested for cross-reactivity against the unmodified protein, so your signal reflects the modified form only. Already know which modification you’re after? Browse our ready-to-use PTM ELISA kits >

Sample Types We Validate

Serum, plasma, and cell culture supernatant are standard. Using samples you provide, we can also validate assays in other matrices, such as CSF, urine, saliva, cell and tissue lysates, and exosomes.

Validation includes spike-recovery and dilution linearity in your matrix. Recovery typically falls within 80-130%.

How It Works

  1. Tell us what you need: Target, modification, species, sample type, and expected concentration range
  2. Feasibility review: Our scientists check antibody and standard availability, then send you a plan and quote within 1-3 business days
  3. Development: We select antibody pairs, prepare standards, and optimize the assay
  4. Validation: Sensitivity, specificity, precision, recovery, dilution linearity, and stability. See our full validation process >
  5. Delivery: You review the validation report, then receive your kit, or we run your samples for you

Typical timeline: 4-7 weeks when validated antibody pairs already exist, longer when new antibodies are needed. Custom development is best suited to larger projects, and we’ll tell you up front whether yours is a fit.

Ready to build your assay? Tell us your target, and we’ll tell you what’s possible.

Request a Custom ELISA Quote
FAQ

Frequently Asked Questions

Still have questions?

Contact Us

Often, yes. It depends on having an antibody that tells the modified form apart from the unmodified one. If none exists, we’ll tell you up front and talk through your options.

It’s simpler. We validate one of our existing ELISA kits in your matrix with spike-recovery and dilution linearity testing, which is usually faster than building a new assay.

Typically 4-7 weeks when we already have validated antibody pairs. New antibodies or modified-protein standards add time, and we’ll give you a timeline with your quote.

Yes. Before we deliver any custom assay, we provide a validation report covering the standard curve, sensitivity, precision, recovery, and dilution linearity.

Our sandwich ELISA kits are evaluated for intra-assay reproducibility and precision by running 2-3 positive control samples in duplicate on a single plate. Inter-assay reproducibility is evaluated by at least 2 independent experiments with 2-3 positive control samples and a full standard curve. Lot-to-lot consistency is tested by comparing calculated concentrations of 2-3 positive control samples with calibration curves of current lot and previous lot.

Recovery is determined by spiking various levels of target protein into biological samples. Dilution linearity is tested by performing 2-fold and 4-fold dilutions of biological samples. Recoveries typically range from 80-130%.

Yes. Send us your samples and we’ll run them in our GLP-compliant lab on kits manufactured under GMP. Learn about ELISA testing services >

We also develop multiplex antibody arrays, bead-based panels, lateral flow assays, and PCR assays. See all assay development services >

Still have questions?

Contact Us

Related Services

ELISA Services

Send us your samples, we’ll send you results. Our scientists run your study in our GLP-compliant lab on RayBio® ELISA kits manufactured under GMP.

Assay Development Services

Need a format beyond ELISA? We develop and validate arrays, lateral flow, PCR, and Western blot assays, from design to validation.

Array Services

Screen hundreds of proteins at once, then confirm your hits by ELISA using the same antibody pairs.

Related Products

PTM ELISA Kits

Ready-to-use ELISA kits for phosphorylated and other post-translationally modified proteins.

ELISA Kits

Analyze and quantify proteins with high-quality ELISA kits and validated antibody pairs, manufactured in our own US-based facilities.

Post-Translational Modification Arrays

Profile phosphorylation and other modifications across many proteins at once before narrowing to a single-target ELISA.

Antibodies

Choose from a wide range of pre-conjugated and unlabeled antibodies raised in a wide variety of species.

Products
  • Multiplex Assays
  • ELISA Kits
  • Proteins and Peptides
  • Antibodies
  • Flow Cytometry
  • Assay Kits
  • Molecular Biology

Sign up to get promotions on your favorite research tools and research updates.

Sign up to Newsletter (opens in new tab)
Services
  • Multiplex Assay Services
  • ELISA Services
  • Antibody Services
  • Custom Protein Services
  • CRO Services
  • Flow Cytometry Services
  • Simoa – Single Molecule Array Services
Resources
  • Manual Protocols
  • Resource Library
  • Learning Center
  • Array Analysis Tools
  • Publications / Citations
  • Publications by RayBiotech Scientists
About Us
  • About RayBiotech
  • Careers
  • Quality Systems
  • Business Partnerships
  • News & Events
  • Reducing Our Emissions
Contact Us
  • Contacts
  • Distributors
  • Certified Service Providers
ISO 13485:2016cGMP
ISO 17025:2017CLIA
©2007-2026 RayBiotech, Inc. All rights reserved.Life Science Web Design By Supreme
  • Privacy Policy
  • Terms & Conditions
  • ISO Certification
  • Risk Free Guarantee
  • Promotions

Your cart is empty.