Membrane-based inflammation cytokine array detects 40 human inflammatory factors.
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Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 40 |
| Recommended Applications | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
|
Eotaxin-1
(CCL11)
|
Eotaxin-2
(MPIF-2/CCL24)
|
GCSF
|
GM-CSF
|
ICAM-1
(CD54)
|
|
IFN-gamma
|
I-309
(TCA-3/CCL1)
|
IL-1 alpha
(IL-1 F1)
|
IL-1 beta
(IL-1 F2)
|
IL-2
|
|
IL-3
|
IL-4
|
IL-6
|
IL-6 R
|
IL-7
|
|
IL-8
(CXCL8)
|
IL-10
|
IL-11
|
IL-12 p40
|
IL-12 p70
|
|
IL-13
|
IL-15
|
IL-16
|
IL-17A
|
IP-10
(CXCL10)
|
|
MCP-1
(CCL2)
|
MCP-2
(CCL8)
|
M-CSF
|
MIG
(CXCL9)
|
MIP-1 alpha
(CCL3)
|
|
MIP-1 beta
(CCL4)
|
MIP-1 delta
(CCL15)
|
RANTES
(CCL5)
|
TGF beta 1
|
TNF alpha
|
|
TNF beta
(TNFSF1B)
|
TNF RI
(TNFRSF1A)
|
TNF RII
(TNFRSF1B)
|
PDGF-BB
|
TIMP-2
|
Typical Data
Kit Components
- Human Inflammation Antibody Array C3 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Additional Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Assay Procedure Summary
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.