Introduction
Mitochondria are central to cellular energy production, metabolism, redox regulation, calcium homeostasis, and apoptosis. Dysregulation of mitochondrial function is closely associated with altered metabolism, oxidative stress, cell death, aging, and a wide range of physiological and pathological conditions.
Product Features
- SYBR Green-based qPCR array for simultaneous expression profiling
- 90 rigorously validated, highly specific primer pairs
- Includes six controls: three reference genes (INC) for normalization, a genomic DNA control (GDC), a positive PCR control (PC), and a negative control (NC)
- Simple workflow — just add cDNA and qPCR master mix
- High-quality results in as little as two hours
Array Content
Simultaneous profiling of 90 validated genes associated with mitochondrial function — membrane polarization and potential, mitochondrial and small-molecule transport, protein import, membrane translocation, fission and fusion, localization, and apoptosis-related processes — plus six on-plate controls.
Format Selection / Instrument Key
Select the array plate format that matches your real-time PCR instrument:
- Plate A: ABI 5700, ABI 7000, ABI 7300, ABI 7500, ABI 7700
- Plate B: ABI 7500 Fast, ABI 7900 HT Fast, ABI StepOnePlus, ABI ViiA 7 Fast, Bio-Rad CFX Connect
- Plate C: Roche LightCycler 96, Roche LightCycler 480 (96-well)
Kit Components
- 96-Well Array Plate(s), preloaded with validated paired primers (2 µL/well)
- 2X qPCR Master Mix with Hot Start Taq
- 96-Well Plate Sealing Film
Storage/Stability
Store the kit at -20°C for up to 12 months prior to opening. Avoid repeated freeze-thaw cycles.
Product Use
For research use only. Not for use in clinical or in vitro diagnostic procedures.
