The RayBio® Mitochondria, Cytosol and Nuclear Fractionation Kit provides a fast and efficient method for enriching mitochondrial, cytosolic, and nuclear protein fractions from cultured cells or tissues.
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Product Description
Specifications
| Size | 25 tests, 50 tests, 100 tests |
| Estimated Lead Time | 1-2 business days |
| Compatible Sample Types | Intact Cells, Tissue |
| Research Area | Mitochondria Function |
| Shipping Type | Blue ice |
| Storage | 4°C |
Introduction
The RayBio® Mitochondria, Cytosol and Nuclear Fractionation Kit provides a fast and efficient method for enriching mitochondrial, cytosolic, and nuclear protein fractions from cultured cells or tissues. The kit uses a specially formulated reagent system to help minimize cross-contamination while maintaining high protein yield from each fraction. The enriched fractions are suitable for a variety of downstream applications, including Western blotting, ELISA, mRNA analysis, and DNA assays. The procedure is simple to perform and can be completed in as little as 2 hours for up to 10 samples.
Product Features
- Suitable for samples from various species
- High protein yield
- Rapid fractionation within 2 hours
Kit Components
| Component | Size / Description |
|---|---|
| Lysis Buffer I | 15 mL |
| Lysis Buffer II | 10 mL |
| Lysis Buffer III | 10 mL |
| Protease Inhibitor Cocktail | Lyophilized powder |
Additional Materials Required
- Pipette tips (barrier tips recommended) and microcentrifuge tubes
- PBS
- Microcentrifuge
- Vortex mixer
- Tissue homogenizer if using tissue samples (Dounce tissue grinder recommended)
- Refrigerated centrifuge with swing out rotor and refrigerated microcentrifuge
Assay Procedure Summary
- Prepare cultured cell or tissue samples as instructed in the manual.
- Prepare all reagents and keep buffers on ice during the procedure.
- Divide the prepared sample into a whole cell extract control and a fractionation sample.
- Sequentially isolate the cytosolic, mitochondrial, and nuclear fractions from the fractionation sample.
- Store the enriched fractions at -80°C until use.
Typical Data
Storage/Stability
The kit can be stored at 4°C for a period of 12 months prior to opening. After Protease Inhibitor Cocktail has been reconstituted, store at -20°C or -80°C.
