Human MMP-1 ELISA

$398.00
ELH-MMP1-1

RayBio® Human MMP-1 ELISA Kit for cell culture supernatants, heparin treated plasma, and serum samples. EDTA and Citrate are not recommended.

Product Features

Sensitivity
8 pg/ml

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Detection Range
8 pg/ml - 18000 pg/ml
Recommended Dilution (serum & plasma)
2 - 10 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-2, - 3, -9, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Species Detected
Human
Protein Name & Synonyms
Interstitial collagenase (EC 3.4.24.7) (Fibroblast collagenase) (Matrix metalloproteinase-1) (MMP-1)
Accession Number
Gene Names
MMP1, CLG
Gene ID
4312

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Antigen Information

Gene Symbol:
  • MMP1

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Array/ELISA Format

Species Detected:
  • Human
Solid Support:
96-well Microplate
Design Principle:
  • Sandwich-based
Quantitative/Semi-Quantitative:
Quantitative
Method of Detection:
Colorimetric
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants

  1. Humbert P., Fanian F., Lihoreau T., et al. Mécano-Stimulation™ of the skin improves sagging score and induces beneficial functional modification of the fibroblasts: clinical, biological, and histological evaluations. Clin Interv Aging. 2015 Feb 2;10:387-403. doi: 10.2147/CIA.S69752
    Species: Human
    Sample Type: Conditioned Media

  2. Garratt L., Sutanto E., Ling K., et al. Matrix metalloproteinase activation by free neutrophil elastase contributes to bronchiectasis progression in early cystic fibrosis. Eur Respir J. 2015 Aug;46(2):384-94. doi: 10.1183/09031936.00212114.
    Species: Human
    Sample Type: BAL Fluid

  3. Markiewicz L., Pytel D., Mucha B., Szymanek K., et al. Altered Expression Levels of MMP1, MMP9, MMP12, TIMP1, and IL-1β as a Risk Factor for the Elevated IOP and Optic Nerve Head Damage in the Primary Open-Angle Glaucoma Patients. BioMed Research International Volume 2015 (2015), Article ID 812503, 8 pages http://dx.doi.org/10.1155/2015/812503
    Species: Human
    Sample Type: Aqueous Humor

  4. Tiraravesit N., Yakaew S., Rukchay R., et al. Artocarpus altilis heartwood extract protects skin against UVB in vitro and in vivo. J Ethnopharmacol. 2015 Dec 4;175:153-62. doi: 10.1016/j.jep.2015.09.023
    Species: Human
    Sample Type: Conditioned Media

  5. Abd-Allah S., El-Shal A., Shalaby S., et al. Influence of Matrix metalloproteinase 1 and 3 genetic variations on susceptibility and severity of juvenile idiopathic arthritis. IUBMB Life. 2015 Dec;67(12):934-42. doi: 10.1002/iub.1446.
    Species: Human
    Sample Type: Serum

  6. Ghodpage P., et al. Influence of phase I periodontal therapy on levels of matrix metalloproteinase 1 and tissue inhibitor of metalloproteinase 1 . The Saudi Dental Journal. Available online 15 July 2014 DOI: 10.1016/j.sdentj.2014.05.003
    Species: Human
    Sample Type: Gingival Crevicular Fluid

  7. Gotoh M., et al. Cyclic phosphatidic acid relieves osteoarthritis symptoms. Gotoh et al. Molecular Pain 2014, 10:52. doi:10.1186/1744-8069-10-52
    Species: Human
    Sample Type: Conditioned Media

  8. Itsarasook K., et al. Artocarpin-enriched extract reverses collagen metabolism in UV-exposed fibroblasts. Biologia 69/7: 943—951, 2014. DOI: 10.2478/s11756-014-0394-4
    Species: Human
    Sample Type: Conditioned Media

  9. Park G., et al. Antioxidant effects of the sarsaparilla viascavenging of reactive oxygen species andinduction of antioxidant enzymes in humandermal fibroblasts. Environmental Toxicology and Pharmacology, Volume 38, Issue 1, July 2014, Pages 305-315
    Species: Human
    Sample Type: Cell Lysate

  10. Popat R., Bhavsar NV., Popat PR. Gingival crevicular fluid levels of Matrix Metalloproteinase-1 (MMP-1) and Tissue Inhibitor of Metalloproteinase-1 (TIMP-1) in periodontal health and disease. Singapore Dent J. 2014 Dec;35:59-64. doi: 10.1016/j.sdj.2014.07.003.
    Species: Human
    Sample Type: Gingival Crevicular Fluid

  11. Rolin G., et al. In vitro study of the impact of mechanical tension on the dermal fibroblast henotype in the context of skin wound healing. Journal of Biomechanics Sept 2014, epub ahead of print. http://dx.doi.org/10.1016/j.jbiomech.2014.07.015
    Species: Human
    Sample Type: Conditioned Media

  12. Angelini PD, Zacarias Fluck MF, Pederson K, et al. Constitutive HER2 signaling promotes breast cancer metastasis through cellular senescence. Cancer Res. 2013;73(1); 450-458.
    Species: Human
    Sample Type: Conditioned Media

  13. Modulation of Cytokine Release and Gene Expression by the Immunosuppressive Domain of gp41 of HIV-1
    Species: Human
    Sample Type: Conditioned Media

  14. Yuan W, Zhao M-D, Yuan F-L, et al. Association of endothelin-1 expression and cartilaginous endplate degeneration in humans. PLoS ONE. 2013;8(4):e60062.
    Species: Human
    Sample Type: Conditioned Media

  15. Kraus D, Winter J, Jepsen S, Jäger A, Meyer R, Deschner J. Interactions of Adiponectin and Lipopolysaccharide from Porphyromonas gingivalis on Human Oral Epithelial Cells. Heimesaat MM, ed. PLoS ONE 2012;7(2):e30716. doi:10.1371/journal.pone.0030716.
    Species: Human
    Sample Type: Conditioned Media

  16. Sundrani DP, Chavan-Gautam PM, Pisal HR, Mehendale SS, Joshi SR (2012) Matrix Metalloproteinase-1 and -9 in Human Placenta during SpontaneousVaginal Delivery and Caesarean Sectioning in Preterm Pregnancy. PLoS ONE 7(1): e29855. doi:10.1371/journal.pone.0029855
    Species: Human
    Sample Type: Tissue Lysate

  17. Park G., Jang D., Oh M. Juglans mandshurica leaf extract protects skin fibroblasts from damage by regulating the oxidative defense system. Biochem Biophys Res Commun. 2012 May 4;421(2):343-8.
    Species: Human
    Sample Type: Conditioned Media

  18. Zheng L., Huang Y., Song W., et al. Fluid shear stress regulates metalloproteinase-1 and 2 in human periodontal ligament cells: involvement of extracellular signal-regulated kinase (ERK) and P38 signaling pathways. J Biomech. 2012 Sep 21;45(14):2368-75. doi: 10.1016/j.jbiomech.2012.07.013
    Species: Human
    Sample Type: Purified Protein

  19. Soydinc H., Sak M., Evliyaoglu O., et al. Maternal plasma prolidase, matrix metalloproteinases 1 and 13, and oxidative stress levels in pregnancies complicated by preterm premature rupture of the membranes and chorioamnionitis. J Turk Ger Gynecol Assoc. 2012 Sep 1;13(3):172-7. doi: 10.5152/jtgga.2012.23.
    Species: Human
    Sample Type: Plasma

  20. Jacob J., Eisemon E., Sheibani-Rad S., et al. Matrix metalloproteinase levels as a marker for rotator cuff tears. Orthopedics. 2012 Apr;35(4):e474-8. doi: 10.3928/01477447-20120327-18.
    Species: Human
    Sample Type: Tissue Lysate

  21. Mahmoud A., Bakir A., Shaban S., et al. Serum TGF-β, Serum MMP-1, and HOMA-IR as Non-Invasive Predictors of Fibrosis in Egyptian Patients with NAFLD. Saudi J Gastroenterol. 2012 Sep-Oct; 18(5): 327-333. doi: 10.4103/1319-3767.101132
    Species: Human
    Sample Type: Serum

  22. Abd-Allah S., Shalaby S., Pasha H., et al. Variation of matrix metalloproteinase 1 and 3 haplotypes and their serum levels in patients with rheumatoid arthritis and osteoarthritis. Genet Test Mol Biomarkers. 2012 Jan;16(1):15-20. doi: 10.1089/gtmb.2011.0003
    Species: Human
    Sample Type: Serum

  23. Decreased Mechanical Properties of Heart Valve Tissue Constructs Cultured in Platelet Lysate as Compared to Fetal Bovine Serum
    Species: Human

  24. Yao H., et al. The PHSCN dendrimer as a more potent inhibitor of human breast cancer cell invasion, extravasation, and lung colony formation. Breast Cancer Res Treat. 2011 Jan;125(2):363-75. doi: 10.1007/s10549-010-0826-y.
    Species: Human

  25. Ariganello, Marianne B., et al. "Macrophage differentiation and polarization on a decellularized pericardial biomaterial." Biomaterials 32.2 (2011): 439-449.
    Species: Human
    Sample Type: Cell Lysate

  26. Ariganello, Marianne B., et al. "Macrophage differentiation and polarization on a decellularized pericardial biomaterial." Biomaterials 32.2 (2011): 439-449.
    Species: Human
    Sample Type: Conditioned Media

  27. Lee YS., et al. Myricetin inhibits IL-1β-induced inflammatory mediators in SW982 human synovial sarcoma cells. Int Immunopharmacol. 2010 Jul;10(7):812-4. doi: 10.1016/j.intimp.2010.04.010
    Species: Human

  28. Upadhyay A, Sharma G, Kivivuori S, Raye W S, Zabihi E, et al. Role of a LIF antagonist in LIF and OSM induced MMP-1, MMP-3, and TIMP-1 expression by primary articular chondrocytes. Cytokine. 2009;46:332-338.
    Species: Porcine
    Sample Type: Conditioned Media

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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