Detects 18 different phosphorylated human and mouse proteins crucial to the AKT Pathway
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Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Estimated Lead Time | 1-2 business days |
| Species | Human, Mouse |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 18 |
| Recommended Applications | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Research Area | Post-Translational Modifications, Phosphorylation, AMPK Signaling, HER/ErbB Signaling, HIF-1 alpha Signaling, IGF Signaling, JAK/STAT Signaling, MAPK Signaling, mTOR Signaling, PI3K-AKT Signaling, TGF-beta Signaling, Wnt-beta-Catenin Signaling, Akt Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
|
Akt (P-Ser473)
(PKB (P-Ser473))
|
AMPKa (P-Thr172)
|
BAD (P-Ser112)
|
4E-BP1 (P-Thr36)
|
ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187)
|
|
GSK3a (P-Ser21)
|
GSK3b (P-Ser9)
|
mTOR (P-Ser2448)
|
p27 (P-Thr198)
|
P53 (P-Ser15)
|
|
P70S6K (P-Thr421/Ser424)
|
PDK1 (P-Ser241)
|
PRAS40 (P-Thr246)
|
PTEN (P-Ser380)
|
Raf-1 (Ser301)
|
|
RPS6 (P-Ser235/Ser236)
|
RSK1 (P-Ser380)
|
RSK2 (P-Ser386)
|
||
Kit Components
- Human and Mouse AKT Pathway Phosphorylation Antibody Array C1 Membranes
- Blocking Buffer
- Detection Antibody Cocktail
- 1,000X HRP-Anti-Rabbit IgG Concentrate
- 20X Wash Buffer I Concentrate
- 20X Wash Buffer II Concentate
- 2X Cell Lysis Buffer Concentrate
- Detection Buffer C
- Detection Buffer D
- 8-Well Incubation Tray
- Protease Inhibitor Cocktail
- 100x Phosphatase Inhibitor Cocktail I
- Phosphatase Inhibitor Cocktail II
- Plastic Sheets
- Array Map Template
- Manual
Additional Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Assay Procedure Summary
- Block membranes
- Incubate with Sample
- Incubate with Detection Antibody Cocktail
- Incubate with HRP-Conjugated anti-IgG
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Typical Data
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
