The RayBiotech Phospho-AKT (S473) ELISA Kit measures phosphorylated AKT (Ser473) and total (pan) AKT in cell and tissue lysates.
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| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Estimated Lead Time | 1-2 business days |
| Species | Human, Mouse, Rat |
| Specificity | The antibody pair provided in this kit recognizes human, mouse and rat Phospho-Akt (pSer473). |
| Compatible Sample Types | Tissue Lysates, Cell Lysates |
| Accession Number | P31749 |
| Gene Id | 391 |
| Gene Symbols | AKT1, PKB, RAC |
| Protein Name / Synonyms | RAC-alpha serine/threonine-protein kinase (EC 2.7.11.1) (Protein kinase B) (PKB) (Protein kinase B alpha) (PKB alpha) (Proto-oncogene c-Akt) (RAC-PK-alpha) |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis, Apoptosis, Post-Translational Modifications, Inflammation, Phosphorylation, mTOR Signaling, PI3K-AKT Signaling, Insulin Signaling, Autophagy, Akt Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
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Phospho-AKT (S473) ELISA Kit
This ELISA worked as well as expected. No complaints here!
Review by Lance, from Columbia University Medical Center, on Jan 13, 2020
This is a highly effective kit to semi-quantitatively measure phosphorylated Akt (Ser473) in lysates in a a 96-well plate format. Easy to read and quite reproducible. I would highly recommend it.
Review by Vinayak, from Moffitt Cancer Center, on Dec 8, 2019
Works well for detection in bone marrow stromal cell lysates
Review by William, on Apr 10, 2018
Phospho-AKT (S473) ELISA Kit