Human Periostin ELISA

RayBio® Human Periostin ELISA Kit for cell culture supernatants, plasma, and serum samples.

Old price: $577.00
$519.30
You save: $57.70 (10%)
ELH-POSTN-1
+

Antigen Information

Accession Number:
  • Q15063
Gene ID:
  • 10631
Gene Symbols:
  • OSF2
  • POSTN
Protein Name & Synonyms:
Periostin (PN) (Osteoblast-specific factor 2) (OSF-2)
Target Species:
  • Human

Assay Format

Detection Range:
80 pg/ml - 60 ng/ml
Recommended Dilution (serum & plasma):
2-20 fold
Sensitivity:
80 pg/ml

Need more sensitivity? Check out the new IQELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa technology.

Specificity:
This ELISA antibody pair detects human Periostin. Other species not determined.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
This Periostin ELISA kit came in with good packaging and in good condition. The directions are easy to follow. Minimum detectable dose is 80 pg/ml, if only it was lower. Also, step 3 in protocol says: "Add 100 μl of each standard (see Reagent Preparation step 3) and sample into appropriate wells. Cover wells and incubate for 2.5 hours at room temperature with gentle shaking." I understand we may want to incubate standards, but incubating SAMPLES at room temperature with NOTHING for 2.5 hours? I don't really get why. Anyways, we got results, which is what matters.

New York Medical College
Write A Review
U.S. Non-U.S.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

Amazon Gift Card Program