Human Tau ELISA Kit

RayBio® Human MAPT (Tau) ELISA Kit for cell culture supernatants, EDTA and/or Citrate treated plasma, and serum samples. Heparin is not recommended.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

$629.00

Human Tau ELISA Kit

RayBio® Human MAPT (Tau) ELISA Kit for cell culture supernatants, EDTA and/or Citrate treated plasma, and serum samples. Heparin is not recommended.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Estimated Lead Time	1-2 business days
Species	Human
Sensitivity	100 pg/ml Need more sensitivity? Check out the new NexaTag^® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa^™ technology.
Detection Range	100 pg/mL - 25,000 pg/mL
Specificity	This ELISA antibody pair detects human Tau. Other species not determined.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum
Recommended Dilution (Serum/Plasma)	2 fold
Accession Number	P10636
Gene Id	4137
Gene Symbols	MAPT, MAPTL, MTBT1, TAU
Protein Name / Synonyms	Microtubule-associated protein tau (Neurofibrillary tangle protein) (Paired helical filament-tau) (PHF-tau)
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Design Principle	Sandwich-based
Method Of Detection	Colorimetric
Research Area	Autophagy
Shipping Type	Blue ice
Storage	≤-20°C

Product Features

Additional Notes

Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Other Materials Required

Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation
100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Protocol Outline

Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Customize This Human Tau ELISA

We manufacture our ELISAs in-house, so we can build a custom version for your study.

Post-translational modifications (PTMs): Detect modified Tau (MAPT), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
Also available: More sensitive platforms such as Simoa^® and NexaTag^®, multiplex panels, bulk lots, and other species when suitable reagents exist

Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.

Learn more about custom ELISA development

Have this product in hand?

Get your lot-specific datasheet at Ray's Docs. The datasheet linked below is our current version and may not match your lot.

Datasheet (ELH-Tau.pdf)

SDS (ELH-Tau.pdf)

Excel (5af824478c7690acd2cf4518aeaae2058a44fa75.xlsm)

Product Features

Additional Notes

Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Other Materials Required

Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation
100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Protocol Outline

Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Storage/Stability

Customize This Human Tau ELISA

We manufacture our ELISAs in-house, so we can build a custom version for your study.

Post-translational modifications (PTMs): Detect modified Tau (MAPT), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
Also available: More sensitive platforms such as Simoa^® and NexaTag^®, multiplex panels, bulk lots, and other species when suitable reagents exist

Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.

Learn more about custom ELISA development

Featured Products

Human Tau ELISA Kit

Human Tau ELISA Kit

Product Description

Specifications

Product Features

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Storage/Stability

Customize This Human Tau ELISA

Documents

Have this product in hand?

Reviews

Ask A Question

Featured Products

Human Tau ELISA KitHuman Tau ELISA Kit

Product Description

Specifications

Related Products

Product Features

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Storage/Stability

Customize This Human Tau ELISA

Documents

Have this product in hand?

Reviews

Great product

Ask A Question

Great product

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