Product Description

Specifications

Size	2 Sample Kit, 4 Sample Kit, 8 Sample Kit
Estimated Lead Time	1-2 business days
Species	Mouse
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Quantitative/Semi-Quantitative	Semi-Quantitative
Number of Targets Detected	97
Solid Support	Membrane
Design Principle	Sandwich-based
Method Of Detection	Chemiluminescence
Shipping Type	Blue ice
Storage	-20°C

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
6Ckine (CCL21) Gene Symbol: CCL21 Accession #: P86792 ELISA Kit: RayBio Mouse 6Ckine ELISA	ALK-1 Gene Symbol: SLPI Accession #: O09081 ELISA Kit: RayBio Mouse ALK-1 ELISA	Amphiregulin Gene Symbol: AREG Accession #: P31955 ELISA Kit: RayBio Mouse AREG ELISA	Axl Gene Symbol: Axl Accession #: Q00993 ELISA Kit: RayBio Mouse Axl ELISA	BLC (CXCL13) Gene Symbol: CXCL13 Accession #: O55038 ELISA Kit: RayBio Mouse BLC ELISA
Cardiotrophin-1 (CT-1) Gene Symbol: CTF1 Accession #: Q60753 ELISA Kit: RayBio Mouse CT-1 ELISA	CD27 (TNFRSF7) Gene Symbol: CD27 Accession #: P41272 ELISA Kit: RayBio Mouse CD27 ELISA	CD27 Ligand (TNFSF7) Gene Symbol: CD70 Accession #: P32970 ELISA Kit: RayBio Mouse CD27 L ELISA	CD30 (TNFRSF8) Gene Symbol: CD30 Accession #: Q60846 ELISA Kit: RayBio Mouse CD30 ELISA	CD30 Ligand (TNFSF8) Gene Symbol: CD30LG Accession #: P32972 ELISA Kit: RayBio Mouse CD30 L ELISA
CD36 (SR-B3) Gene Symbol: CD36 Accession #: Q08857 ELISA Kit: RayBio Mouse CD36 ELISA	CTLA-4 (CD152) Gene Symbol: Ctla4 Accession #: Q9QZZ7 ELISA Kit: RayBio Mouse CTLA-4 ELISA	CXCL16 Gene Symbol: CXCL16 Accession #: Q5F2D5 ELISA Kit: RayBio Mouse CXCL16 ELISA	Decorin Gene Symbol: DCN Accession #: P28654 ELISA Kit: RayBio Mouse Decorin ELISA	DKK-1 Gene Symbol: DKK1 Accession #: Q80UL5 ELISA Kit: RayBio Mouse Dkk-1 ELISA
E-Cadherin Gene Symbol: CDH1 Accession #: P09803 ELISA Kit: RayBio Mouse E-Cadherin ELISA	EGF Gene Symbol: EGF Accession #: E9QNX6 ELISA Kit: RayBio Mouse EGF ELISA	Eotaxin-1 (CCL11) Gene Symbol: CCL11 Accession #: P48298 ELISA Kit: RayBio Mouse Eotaxin-1 ELISA	Eotaxin-2 (MPIF-2/CCL24) Gene Symbol: Ccl24 Accession #: Q9JKC0 ELISA Kit: RayBio Mouse Eotaxin-2 ELISA	Epigen Gene Symbol: EPGN Accession #: Q8CEX5 ELISA Kit: RayBio Mouse Epigen ELISA
E-Selectin Gene Symbol: SELE Accession #: Q00690 ELISA Kit: RayBio Mouse E-Selectin ELISA	Fas Ligand (TNFSF6) Gene Symbol: FASLG Accession #: P41047 ELISA Kit: RayBio Mouse Fas L ELISA	Fc gamma RIIB (CD32b) Gene Symbol: FCGR2B Accession #: P08101 ELISA Kit: RayBio Mouse Fc-gamma-RIIb ELISA	Flt-3 Ligand Gene Symbol: FLT3LG Accession #: P49772 ELISA Kit: RayBio Mouse Flt-3L ELISA	Fractalkine (CX3CL1) Gene Symbol: CX3CL1 Accession #: O35188 ELISA Kit: RayBio Mouse Fractalkine ELISA
Galectin-1 Gene Symbol: LGALS1 Accession #: P17601 ELISA Kit: RayBio Mouse Galectin-1 ELISA	Gas 6 Gene Symbol: Gas6 Accession #: Q99K57 ELISA Kit: RayBio Mouse GAS6 ELISA	GCSF Gene Symbol: CSF3 Accession #: P09920 ELISA Kit: RayBio Mouse G-CSF ELISA	GITR (TNFRSF18) Gene Symbol: TNFRSF18 Accession #: Q9JKR2 ELISA Kit: RayBio Mouse GITR ELISA	GITR Ligand (TNFSF18) Gene Symbol: TNFSF18 Accession #: Q7TS55 ELISA Kit: RayBio Mouse GITR L ELISA
Granzyme B Gene Symbol: GZMB Accession #: Q3V2P1 ELISA Kit: RayBio Mouse Granzyme B ELISA	HAI-1 Gene Symbol: SPINT1 Accession #: Q9R097 ELISA Kit: RayBio Mouse HAI-1 ELISA	HGF Gene Symbol: HGF Accession #: Q53WS5 ELISA Kit: RayBio Mouse HGF ELISA	IFN-gamma Gene Symbol: IFNG Accession #: P01580 ELISA Kit: RayBio Mouse IFN-gamma ELISA	IGFBP-5 Gene Symbol: IGFBP5 Accession #: Q07079 ELISA Kit: RayBio Mouse IGFBP-5 ELISA
IGFBP-6 Gene Symbol: IGFBP6 Accession #: Q91X24 ELISA Kit: RayBio Mouse IGFBP-6 ELISA	IGF-2 Gene Symbol: Igf2 Accession #: P09535 ELISA Kit: RayBio Mouse IGF-2 ELISA	IL-1 alpha (IL-1 F1) Gene Symbol: IL1A Accession #: P01582 ELISA Kit: RayBio Mouse IL-1 alpha ELISA	IL-1 beta (IL-1 F2) Gene Symbol: IL1B Accession #: P10749 ELISA Kit: RayBio Mouse IL-1 beta ELISA	IL-1 ra (IL-1 F3) Gene Symbol: IL1RN Accession #: P25085 ELISA Kit: RayBio Mouse IL-1 RA ELISA
IL-2 Gene Symbol: IL2 Accession #: Q791T3 ELISA Kit: RayBio Mouse IL-2 ELISA	IL-2 R alpha Gene Symbol: IL2RA Accession #: Q61731 ELISA Kit: RayBio Mouse IL-2 R alpha ELISA	IL-3 Gene Symbol: IL3 Accession #: P01586 ELISA Kit: RayBio Mouse IL-3 ELISA	IL-4 Gene Symbol: IL4 Accession #: P07750 ELISA Kit: RayBio Mouse IL-4 ELISA	IL-5 Gene Symbol: IL5 Accession #: P04401 ELISA Kit: RayBio Mouse IL-5 ELISA
IL-6 Gene Symbol: IL6 Accession #: P08505 ELISA Kit: RayBio Mouse IL-6 ELISA	IL-9 Gene Symbol: IL9 Accession #: P15247 ELISA Kit: RayBio Mouse IL-9 ELISA	IL-10 Gene Symbol: IL10 Accession #: Q0VBJ1 ELISA Kit: RayBio Mouse IL-10 ELISA	IL-11 Gene Symbol: IL11 Accession #: P47873 ELISA Kit: RayBio Mouse IL-11 ELISA	IL-12 p40 Gene Symbol: IL12B Accession #: P43432
IL-12 p70 Gene Symbol: IL12A Accession #: P43431	IL-13 Gene Symbol: IL13 Accession #: P20109 ELISA Kit: RayBio Mouse IL-13 ELISA	IL-15 Gene Symbol: IL15 Accession #: Q1AHQ7 ELISA Kit: RayBio Mouse IL-15 ELISA	IL-17A Gene Symbol: IL17A Accession #: Q60971 ELISA Kit: RayBio Mouse IL-17A ELISA	IL-17 RB Gene Symbol: IL17RB Accession #: Q9JIP3 ELISA Kit: RayBio Mouse IL-17B R ELISA
IL-17E (IL-25) Gene Symbol: IL25 Accession #: Q8VHH8 ELISA Kit: RayBio Mouse IL-17E ELISA	IL-17F Gene Symbol: IL17F Accession #: Q7TNI7 ELISA Kit: RayBio Mouse IL-17F ELISA	IL-20 Gene Symbol: IL20 Accession #: Q9JKV9 ELISA Kit: RayBio Mouse IL-20 ELISA	IL-21 Gene Symbol: IL21 Accession #: E9PX58 ELISA Kit: RayBio Mouse IL-21 ELISA	I-TAC (CXCL11) Gene Symbol: CXCL11 Accession #: Q9JHH5 ELISA Kit: RayBio Mouse I-TAC ELISA
JAM-A (CD321/F11R) Gene Symbol: F11R Accession #: Q8VC39 ELISA Kit: RayBio Mouse JAM-A ELISA	KC (CXCL1) Gene Symbol: CXCL1 Accession #: P12850 ELISA Kit: RayBio Mouse KC ELISA	Leptin Gene Symbol: LEP Accession #: P41160 ELISA Kit: RayBio Mouse Leptin ELISA	Leptin R Gene Symbol: LEPR Accession #: Q61215 ELISA Kit: RayBio Mouse Leptin R ELISA	L-Selectin (CD62L) Gene Symbol: SELL Accession #: Q3TCF3 ELISA Kit: RayBio Mouse L-Selectin ELISA
Lungkine (CXCL15) Gene Symbol: CXCL15 Accession #: Q3UQ15 ELISA Kit: RayBio Mouse CXCL15 ELISA	MAdCAM-1 Gene Symbol: MADCAM1 Accession #: Q61278 ELISA Kit: RayBio Mouse MAdCAM-1 ELISA	MCP-1 (CCL2) Gene Symbol: CCL2 Accession #: Q9QYD7 ELISA Kit: RayBio Mouse MCP-1 ELISA	MDC (CCL22) Gene Symbol: CCL22 Accession #: O88430 ELISA Kit: RayBio Mouse MDC ELISA	MFG-E8 Gene Symbol: Mfge8 Accession #: Q9R1X9 ELISA Kit: RayBio Mouse MFG-E8 ELISA
MIG (CXCL9) Gene Symbol: CXCL9 Accession #: P18340 ELISA Kit: RayBio Mouse MIG ELISA	MIP-1 alpha (CCL3) Gene Symbol: CCL3 Accession #: P14096 ELISA Kit: RayBio Mouse MIP-1 alpha ELISA	MIP-1 gamma Gene Symbol: Ccl9 Accession #: Q5QNW2 ELISA Kit: RayBio Mouse MIP-1 gamma ELISA	MIP-2 Gene Symbol: Cxcl2 Accession #: P10889 ELISA Kit: RayBio Mouse MIP-2 ELISA	MIP-3 alpha (CCL20) Gene Symbol: CCL20 Accession #: O89093 ELISA Kit: RayBio Mouse MIP-3 alpha ELISA
MIP-3 beta (CCL19) Gene Symbol: CCL19 Accession #: O70460 ELISA Kit: RayBio Mouse MIP-3 beta ELISA	MMP-2 Gene Symbol: MMP2 Accession #: P33434 ELISA Kit: RayBio Mouse MMP-2 ELISA	Osteopontin (SPP1) Gene Symbol: SPP1 Accession #: P19008 ELISA Kit: RayBio Mouse OPN ELISA	Osteoprotegerin (TNFRSF11B) Gene Symbol: TNFRSF11B Accession #: O70202 ELISA Kit: RayBio Mouse OPG ELISA	Prolactin Gene Symbol: PRL Accession #: P06879 ELISA Kit: RayBio Mouse Prolactin ELISA
Pro-MMP-9 Gene Symbol: Mmp9 Accession #: Q80XI8 ELISA Kit: RayBio Mouse pro-MMP-9 ELISA	RANTES (CCL5) Gene Symbol: CCL5 Accession #: P30882 ELISA Kit: RayBio Mouse RANTES ELISA	SCF Gene Symbol: KITLG Accession #: P20826 ELISA Kit: RayBio Mouse SCF ELISA	TNF RI (TNFRSF1A) Gene Symbol: TNFRSF1A Accession #: P25118 ELISA Kit: RayBio Mouse sTNFRI ELISA	TNF RII (TNFRSF1B) Gene Symbol: TNFRSF1B Accession #: O88734 ELISA Kit: RayBio Mouse sTNFRII ELISA
TACI (TNFRSF13B) Gene Symbol: TNFRSF13B Accession #: Q9DBZ3 ELISA Kit: RayBio Mouse TACI ELISA	TARC (CCL17) Gene Symbol: CCL17 Accession #: Q9WUZ6 ELISA Kit: RayBio Mouse TARC ELISA	TNF alpha Gene Symbol: TNF Accession #: Q62326 ELISA Kit: RayBio Mouse TNF-alpha ELISA	Thrombopoietin (TPO) Gene Symbol: THPO Accession #: P40226 ELISA Kit: RayBio Mouse TPO ELISA	TRANCE (TNFSF11) Gene Symbol: TNFSF11 Accession #: Q9R1Y0 ELISA Kit: RayBio Mouse TRANCE ELISA
TROY (TNFRSF19) Gene Symbol: TNFRSF19 Accession #: Q80T13 ELISA Kit: RayBio Mouse TROY ELISA	TWEAK R (TNFRSF12) Gene Symbol: TNFRSF12A Accession #: Q9CR75 ELISA Kit: RayBio Mouse TWEAK R ELISA	VCAM-1 (CD106) Gene Symbol: VCAM1 Accession #: P29533 ELISA Kit: RayBio Mouse VCAM-1 ELISA	VEGF-A Gene Symbol: VEGFA Accession #: Q00731 ELISA Kit: RayBio Mouse VEGF-A ELISA	VEGFR1 Gene Symbol: Flt1 Accession #: Q61517 ELISA Kit: RayBio Mouse VEGF R1 ELISA
VEGFR3 Gene Symbol: FLT4 Accession #: P35917 ELISA Kit: RayBio Mouse VEGFR-3 ELISA	VEGF-D Gene Symbol: FIGF Accession #: A2AIH3

Species Detected

Mouse

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Mouse Cytokine Antibody Array C6 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
4-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE's ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
6Ckine (CCL21) Gene Symbol: CCL21 Accession #: P86792 ELISA Kit: RayBio Mouse 6Ckine ELISA	ALK-1 Gene Symbol: SLPI Accession #: O09081 ELISA Kit: RayBio Mouse ALK-1 ELISA	Amphiregulin Gene Symbol: AREG Accession #: P31955 ELISA Kit: RayBio Mouse AREG ELISA	Axl Gene Symbol: Axl Accession #: Q00993 ELISA Kit: RayBio Mouse Axl ELISA	BLC (CXCL13) Gene Symbol: CXCL13 Accession #: O55038 ELISA Kit: RayBio Mouse BLC ELISA
Cardiotrophin-1 (CT-1) Gene Symbol: CTF1 Accession #: Q60753 ELISA Kit: RayBio Mouse CT-1 ELISA	CD27 (TNFRSF7) Gene Symbol: CD27 Accession #: P41272 ELISA Kit: RayBio Mouse CD27 ELISA	CD27 Ligand (TNFSF7) Gene Symbol: CD70 Accession #: P32970 ELISA Kit: RayBio Mouse CD27 L ELISA	CD30 (TNFRSF8) Gene Symbol: CD30 Accession #: Q60846 ELISA Kit: RayBio Mouse CD30 ELISA	CD30 Ligand (TNFSF8) Gene Symbol: CD30LG Accession #: P32972 ELISA Kit: RayBio Mouse CD30 L ELISA
CD36 (SR-B3) Gene Symbol: CD36 Accession #: Q08857 ELISA Kit: RayBio Mouse CD36 ELISA	CTLA-4 (CD152) Gene Symbol: Ctla4 Accession #: Q9QZZ7 ELISA Kit: RayBio Mouse CTLA-4 ELISA	CXCL16 Gene Symbol: CXCL16 Accession #: Q5F2D5 ELISA Kit: RayBio Mouse CXCL16 ELISA	Decorin Gene Symbol: DCN Accession #: P28654 ELISA Kit: RayBio Mouse Decorin ELISA	DKK-1 Gene Symbol: DKK1 Accession #: Q80UL5 ELISA Kit: RayBio Mouse Dkk-1 ELISA
E-Cadherin Gene Symbol: CDH1 Accession #: P09803 ELISA Kit: RayBio Mouse E-Cadherin ELISA	EGF Gene Symbol: EGF Accession #: E9QNX6 ELISA Kit: RayBio Mouse EGF ELISA	Eotaxin-1 (CCL11) Gene Symbol: CCL11 Accession #: P48298 ELISA Kit: RayBio Mouse Eotaxin-1 ELISA	Eotaxin-2 (MPIF-2/CCL24) Gene Symbol: Ccl24 Accession #: Q9JKC0 ELISA Kit: RayBio Mouse Eotaxin-2 ELISA	Epigen Gene Symbol: EPGN Accession #: Q8CEX5 ELISA Kit: RayBio Mouse Epigen ELISA
E-Selectin Gene Symbol: SELE Accession #: Q00690 ELISA Kit: RayBio Mouse E-Selectin ELISA	Fas Ligand (TNFSF6) Gene Symbol: FASLG Accession #: P41047 ELISA Kit: RayBio Mouse Fas L ELISA	Fc gamma RIIB (CD32b) Gene Symbol: FCGR2B Accession #: P08101 ELISA Kit: RayBio Mouse Fc-gamma-RIIb ELISA	Flt-3 Ligand Gene Symbol: FLT3LG Accession #: P49772 ELISA Kit: RayBio Mouse Flt-3L ELISA	Fractalkine (CX3CL1) Gene Symbol: CX3CL1 Accession #: O35188 ELISA Kit: RayBio Mouse Fractalkine ELISA
Galectin-1 Gene Symbol: LGALS1 Accession #: P17601 ELISA Kit: RayBio Mouse Galectin-1 ELISA	Gas 6 Gene Symbol: Gas6 Accession #: Q99K57 ELISA Kit: RayBio Mouse GAS6 ELISA	GCSF Gene Symbol: CSF3 Accession #: P09920 ELISA Kit: RayBio Mouse G-CSF ELISA	GITR (TNFRSF18) Gene Symbol: TNFRSF18 Accession #: Q9JKR2 ELISA Kit: RayBio Mouse GITR ELISA	GITR Ligand (TNFSF18) Gene Symbol: TNFSF18 Accession #: Q7TS55 ELISA Kit: RayBio Mouse GITR L ELISA
Granzyme B Gene Symbol: GZMB Accession #: Q3V2P1 ELISA Kit: RayBio Mouse Granzyme B ELISA	HAI-1 Gene Symbol: SPINT1 Accession #: Q9R097 ELISA Kit: RayBio Mouse HAI-1 ELISA	HGF Gene Symbol: HGF Accession #: Q53WS5 ELISA Kit: RayBio Mouse HGF ELISA	IFN-gamma Gene Symbol: IFNG Accession #: P01580 ELISA Kit: RayBio Mouse IFN-gamma ELISA	IGFBP-5 Gene Symbol: IGFBP5 Accession #: Q07079 ELISA Kit: RayBio Mouse IGFBP-5 ELISA
IGFBP-6 Gene Symbol: IGFBP6 Accession #: Q91X24 ELISA Kit: RayBio Mouse IGFBP-6 ELISA	IGF-2 Gene Symbol: Igf2 Accession #: P09535 ELISA Kit: RayBio Mouse IGF-2 ELISA	IL-1 alpha (IL-1 F1) Gene Symbol: IL1A Accession #: P01582 ELISA Kit: RayBio Mouse IL-1 alpha ELISA	IL-1 beta (IL-1 F2) Gene Symbol: IL1B Accession #: P10749 ELISA Kit: RayBio Mouse IL-1 beta ELISA	IL-1 ra (IL-1 F3) Gene Symbol: IL1RN Accession #: P25085 ELISA Kit: RayBio Mouse IL-1 RA ELISA
IL-2 Gene Symbol: IL2 Accession #: Q791T3 ELISA Kit: RayBio Mouse IL-2 ELISA	IL-2 R alpha Gene Symbol: IL2RA Accession #: Q61731 ELISA Kit: RayBio Mouse IL-2 R alpha ELISA	IL-3 Gene Symbol: IL3 Accession #: P01586 ELISA Kit: RayBio Mouse IL-3 ELISA	IL-4 Gene Symbol: IL4 Accession #: P07750 ELISA Kit: RayBio Mouse IL-4 ELISA	IL-5 Gene Symbol: IL5 Accession #: P04401 ELISA Kit: RayBio Mouse IL-5 ELISA
IL-6 Gene Symbol: IL6 Accession #: P08505 ELISA Kit: RayBio Mouse IL-6 ELISA	IL-9 Gene Symbol: IL9 Accession #: P15247 ELISA Kit: RayBio Mouse IL-9 ELISA	IL-10 Gene Symbol: IL10 Accession #: Q0VBJ1 ELISA Kit: RayBio Mouse IL-10 ELISA	IL-11 Gene Symbol: IL11 Accession #: P47873 ELISA Kit: RayBio Mouse IL-11 ELISA	IL-12 p40 Gene Symbol: IL12B Accession #: P43432
IL-12 p70 Gene Symbol: IL12A Accession #: P43431	IL-13 Gene Symbol: IL13 Accession #: P20109 ELISA Kit: RayBio Mouse IL-13 ELISA	IL-15 Gene Symbol: IL15 Accession #: Q1AHQ7 ELISA Kit: RayBio Mouse IL-15 ELISA	IL-17A Gene Symbol: IL17A Accession #: Q60971 ELISA Kit: RayBio Mouse IL-17A ELISA	IL-17 RB Gene Symbol: IL17RB Accession #: Q9JIP3 ELISA Kit: RayBio Mouse IL-17B R ELISA
IL-17E (IL-25) Gene Symbol: IL25 Accession #: Q8VHH8 ELISA Kit: RayBio Mouse IL-17E ELISA	IL-17F Gene Symbol: IL17F Accession #: Q7TNI7 ELISA Kit: RayBio Mouse IL-17F ELISA	IL-20 Gene Symbol: IL20 Accession #: Q9JKV9 ELISA Kit: RayBio Mouse IL-20 ELISA	IL-21 Gene Symbol: IL21 Accession #: E9PX58 ELISA Kit: RayBio Mouse IL-21 ELISA	I-TAC (CXCL11) Gene Symbol: CXCL11 Accession #: Q9JHH5 ELISA Kit: RayBio Mouse I-TAC ELISA
JAM-A (CD321/F11R) Gene Symbol: F11R Accession #: Q8VC39 ELISA Kit: RayBio Mouse JAM-A ELISA	KC (CXCL1) Gene Symbol: CXCL1 Accession #: P12850 ELISA Kit: RayBio Mouse KC ELISA	Leptin Gene Symbol: LEP Accession #: P41160 ELISA Kit: RayBio Mouse Leptin ELISA	Leptin R Gene Symbol: LEPR Accession #: Q61215 ELISA Kit: RayBio Mouse Leptin R ELISA	L-Selectin (CD62L) Gene Symbol: SELL Accession #: Q3TCF3 ELISA Kit: RayBio Mouse L-Selectin ELISA
Lungkine (CXCL15) Gene Symbol: CXCL15 Accession #: Q3UQ15 ELISA Kit: RayBio Mouse CXCL15 ELISA	MAdCAM-1 Gene Symbol: MADCAM1 Accession #: Q61278 ELISA Kit: RayBio Mouse MAdCAM-1 ELISA	MCP-1 (CCL2) Gene Symbol: CCL2 Accession #: Q9QYD7 ELISA Kit: RayBio Mouse MCP-1 ELISA	MDC (CCL22) Gene Symbol: CCL22 Accession #: O88430 ELISA Kit: RayBio Mouse MDC ELISA	MFG-E8 Gene Symbol: Mfge8 Accession #: Q9R1X9 ELISA Kit: RayBio Mouse MFG-E8 ELISA
MIG (CXCL9) Gene Symbol: CXCL9 Accession #: P18340 ELISA Kit: RayBio Mouse MIG ELISA	MIP-1 alpha (CCL3) Gene Symbol: CCL3 Accession #: P14096 ELISA Kit: RayBio Mouse MIP-1 alpha ELISA	MIP-1 gamma Gene Symbol: Ccl9 Accession #: Q5QNW2 ELISA Kit: RayBio Mouse MIP-1 gamma ELISA	MIP-2 Gene Symbol: Cxcl2 Accession #: P10889 ELISA Kit: RayBio Mouse MIP-2 ELISA	MIP-3 alpha (CCL20) Gene Symbol: CCL20 Accession #: O89093 ELISA Kit: RayBio Mouse MIP-3 alpha ELISA
MIP-3 beta (CCL19) Gene Symbol: CCL19 Accession #: O70460 ELISA Kit: RayBio Mouse MIP-3 beta ELISA	MMP-2 Gene Symbol: MMP2 Accession #: P33434 ELISA Kit: RayBio Mouse MMP-2 ELISA	Osteopontin (SPP1) Gene Symbol: SPP1 Accession #: P19008 ELISA Kit: RayBio Mouse OPN ELISA	Osteoprotegerin (TNFRSF11B) Gene Symbol: TNFRSF11B Accession #: O70202 ELISA Kit: RayBio Mouse OPG ELISA	Prolactin Gene Symbol: PRL Accession #: P06879 ELISA Kit: RayBio Mouse Prolactin ELISA
Pro-MMP-9 Gene Symbol: Mmp9 Accession #: Q80XI8 ELISA Kit: RayBio Mouse pro-MMP-9 ELISA	RANTES (CCL5) Gene Symbol: CCL5 Accession #: P30882 ELISA Kit: RayBio Mouse RANTES ELISA	SCF Gene Symbol: KITLG Accession #: P20826 ELISA Kit: RayBio Mouse SCF ELISA	TNF RI (TNFRSF1A) Gene Symbol: TNFRSF1A Accession #: P25118 ELISA Kit: RayBio Mouse sTNFRI ELISA	TNF RII (TNFRSF1B) Gene Symbol: TNFRSF1B Accession #: O88734 ELISA Kit: RayBio Mouse sTNFRII ELISA
TACI (TNFRSF13B) Gene Symbol: TNFRSF13B Accession #: Q9DBZ3 ELISA Kit: RayBio Mouse TACI ELISA	TARC (CCL17) Gene Symbol: CCL17 Accession #: Q9WUZ6 ELISA Kit: RayBio Mouse TARC ELISA	TNF alpha Gene Symbol: TNF Accession #: Q62326 ELISA Kit: RayBio Mouse TNF-alpha ELISA	Thrombopoietin (TPO) Gene Symbol: THPO Accession #: P40226 ELISA Kit: RayBio Mouse TPO ELISA	TRANCE (TNFSF11) Gene Symbol: TNFSF11 Accession #: Q9R1Y0 ELISA Kit: RayBio Mouse TRANCE ELISA
TROY (TNFRSF19) Gene Symbol: TNFRSF19 Accession #: Q80T13 ELISA Kit: RayBio Mouse TROY ELISA	TWEAK R (TNFRSF12) Gene Symbol: TNFRSF12A Accession #: Q9CR75 ELISA Kit: RayBio Mouse TWEAK R ELISA	VCAM-1 (CD106) Gene Symbol: VCAM1 Accession #: P29533 ELISA Kit: RayBio Mouse VCAM-1 ELISA	VEGF-A Gene Symbol: VEGFA Accession #: Q00731 ELISA Kit: RayBio Mouse VEGF-A ELISA	VEGFR1 Gene Symbol: Flt1 Accession #: Q61517 ELISA Kit: RayBio Mouse VEGF R1 ELISA
VEGFR3 Gene Symbol: FLT4 Accession #: P35917 ELISA Kit: RayBio Mouse VEGFR-3 ELISA	VEGF-D Gene Symbol: FIGF Accession #: A2AIH3

Species Detected

Mouse

Application Notes

Suggested Application

Kit Components

Mouse Cytokine Antibody Array C6 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
4-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE's ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

Excellent antibody microarray
Rating
Sample type: Mouse lung bronchoalveolar lavage (BAL) Experiments: BAL was harvested from mice in different conditions (naive versus infected) and levels of cytokines and growth factors were measured using RayBio C6 array (Lot: 8272470908). Results: Key cytokines and chemokines important for protection from infection were highly induced after infection. Interestingly, some other factors that had not been expected were also detected and their levels were significantly changed, providing new insight into lung peripheral immune responses to influenza infection. Benefit of this product: This array covers a large number of factors. It is sensitive and easy-to-use. Highly recommended.
Review by Kihong Lim, PhD, from University of Rochester Medical Center, on Nov 1, 2024

Featured Products

Mouse Cytokine Array C6

Product Description

Specifications

Product Features

Target Names

Species Detected

Application Notes

Storage/Stability

Have this product in hand?

Featured Products

Mouse Cytokine Array C6

Product Description

Specifications

Product Features

Target Names

Species Detected

Application Notes

Storage/Stability

Have this product in hand?

Excellent antibody microarray

Excellent antibody microarray

Mouse Cytokine Array C6

Mouse Cytokine Array C6

Featured Products

Mouse Cytokine Array C6

Product Description

Specifications

Product Features

Target Names

Species Detected

Application Notes

Storage/Stability

Documents

Have this product in hand?

Citations

Reviews

Ask A Question

Featured Products

Mouse Cytokine Array C6

Product Description

Specifications

Product Features

Target Names

Species Detected

Application Notes

Storage/Stability

Documents

Have this product in hand?

Citations

Reviews

Excellent antibody microarray

Ask A Question

Excellent antibody microarray

Mouse Cytokine Array C6

Mouse Cytokine Array C6