1-888-494-8555

Home/Search results/Products/Arrays/Antibody Arrays/Quantibody: Quantitative Multiplex ELISA Arrays/Cytokine Arrays/Mouse Cytokine Array Q1000

Manual / SDS

Description
Citations
Reviews
Files

Antigen Information

Gene Symbols:

AXL | CCL1 | CCL11 | CCL12 | CCL17 | CCL2 | CCL20 | CCL22 | CCL24 | CCL3 View more

Assay Format

Number of Targets Detected:

Species Detected:

Mouse

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Other Body Fluids
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Fluorescence Laser Scanner

Quantitative/Semi-Quantitative:

Quantitative

Solid Support:

Glass Slide

Product Specifications

Size:

1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Product Features

More cost-effective than traditional ELISA
High specificity and system reproducibility
Suitable for diverse sample types
Low sample volume requirement: 100 µL or less
Get results same day (6-hour processing time)
Well-suited for high throughput assays
Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
Amphiregulin Gene Symbol: AREG Accession #: P31955 ELISA Kit: RayBio Mouse AREG ELISA	Axl Gene Symbol: Axl Accession #: Q00993 ELISA Kit: RayBio Mouse Axl ELISA	bFGF Gene Symbol: FGF2 Accession #: P15655 ELISA Kit: RayBio Mouse bFGF ELISA	BLC (CXCL13) Gene Symbol: CXCL13 Accession #: O55038 ELISA Kit: RayBio Mouse BLC ELISA	CD27 Ligand (TNFSF7) Gene Symbol: CD70 Accession #: P32970 ELISA Kit: RayBio Mouse CD27 L ELISA
CD30 (TNFRSF8) Gene Symbol: CD30 Accession #: Q60846 ELISA Kit: RayBio Mouse CD30 ELISA	CD30 Ligand (TNFSF8) Gene Symbol: CD30LG Accession #: P32972 ELISA Kit: RayBio Mouse CD30 L ELISA	CD40 (TNFRSF5) Gene Symbol: CD40 Accession #: Q99NE2 ELISA Kit: RayBio Mouse CD40 ELISA	CXCL16 Gene Symbol: CXCL16 Accession #: Q5F2D5 ELISA Kit: RayBio Mouse CXCL16 ELISA	EGF Gene Symbol: EGF Accession #: E9QNX6 ELISA Kit: RayBio Mouse EGF ELISA
Eotaxin-1 (CCL11) Gene Symbol: CCL11 Accession #: P48298 ELISA Kit: RayBio Mouse Eotaxin-1 ELISA	Eotaxin-2 (MPIF-2/CCL24) Gene Symbol: Ccl24 Accession #: Q9JKC0 ELISA Kit: RayBio Mouse Eotaxin-2 ELISA	E-Selectin Gene Symbol: SELE Accession #: Q00690 ELISA Kit: RayBio Mouse E-Selectin ELISA	Fas Ligand (TNFSF6) Gene Symbol: FASLG Accession #: P41047 ELISA Kit: RayBio Mouse Fas L ELISA	Fractalkine (CX3CL1) Gene Symbol: CX3CL1 Accession #: O35188 ELISA Kit: RayBio Mouse Fractalkine ELISA
GCSF Gene Symbol: CSF3 Accession #: P09920 ELISA Kit: RayBio Mouse G-CSF ELISA	GITR (TNFRSF18) Gene Symbol: TNFRSF18 Accession #: Q9JKR2 ELISA Kit: RayBio Mouse GITR ELISA	GM-CSF Gene Symbol: CSF2 Accession #: P26955 ELISA Kit: RayBio Mouse GM-CSF ELISA	HGF Gene Symbol: HGF Accession #: Q53WS5 ELISA Kit: RayBio Mouse HGF ELISA	I-309 (TCA-3/CCL1) Gene Symbol: CCL1 Accession #: P14098 ELISA Kit: RayBio Mouse TCA-3 ELISA
ICAM-1 (CD54) Gene Symbol: ICAM1 Accession #: P13597 ELISA Kit: RayBio Mouse ICAM-1 ELISA	IFN-gamma Gene Symbol: IFNG Accession #: P01580 ELISA Kit: RayBio Mouse IFN-gamma ELISA	IGF-1 Gene Symbol: IGF1 Accession #: P05018 ELISA Kit: RayBio Mouse IGF-1 ELISA	IGFBP-2 Gene Symbol: IGFBP2 Accession #: P47877 ELISA Kit: RayBio Mouse IGFBP-2 ELISA	IGFBP-3 Gene Symbol: IGFBP3 Accession #: P47878 ELISA Kit: RayBio Mouse IGFBP-3 ELISA
IGFBP-5 Gene Symbol: IGFBP5 Accession #: Q07079 ELISA Kit: RayBio Mouse IGFBP-5 ELISA	IGFBP-6 Gene Symbol: IGFBP6 Accession #: Q91X24 ELISA Kit: RayBio Mouse IGFBP-6 ELISA	IL-1 alpha (IL-1 F1) Gene Symbol: IL1A Accession #: P01582 ELISA Kit: RayBio Mouse IL-1 alpha ELISA	IL-1 beta (IL-1 F2) Gene Symbol: IL1B Accession #: P10749 ELISA Kit: RayBio Mouse IL-1 beta ELISA	IL-1 ra (IL-1 F3) Gene Symbol: IL1RN Accession #: P25085 ELISA Kit: RayBio Mouse IL-1 RA ELISA
IL-10 Gene Symbol: IL10 Accession #: Q0VBJ1 ELISA Kit: RayBio Mouse IL-10 ELISA	IL-12 p40 Gene Symbol: IL12B Accession #: P43432	IL-12 p70 Gene Symbol: IL12A Accession #: P43431	IL-13 Gene Symbol: IL13 Accession #: P20109 ELISA Kit: RayBio Mouse IL-13 ELISA	IL-15 Gene Symbol: IL15 Accession #: Q1AHQ7 ELISA Kit: RayBio Mouse IL-15 ELISA
IL-17A Gene Symbol: IL17A Accession #: Q60971 ELISA Kit: RayBio Mouse IL-17A ELISA	IL-17E (IL-25) Gene Symbol: IL25 Accession #: Q8VHH8 ELISA Kit: RayBio Mouse IL-17E ELISA	IL-17F Gene Symbol: IL17F Accession #: Q7TNI7 ELISA Kit: RayBio Mouse IL-17F ELISA	IL-2 Gene Symbol: IL2 Accession #: Q791T3 ELISA Kit: RayBio Mouse IL-2 ELISA	IL-2 R alpha Gene Symbol: IL2RA Accession #: Q61731 ELISA Kit: RayBio Mouse IL-2 R alpha ELISA
IL-20 Gene Symbol: IL20 Accession #: Q9JKV9 ELISA Kit: RayBio Mouse IL-20 ELISA	IL-21 Gene Symbol: IL21 Accession #: E9PX58 ELISA Kit: RayBio Mouse IL-21 ELISA	IL-23 p19 Gene Symbol: IL23A Accession #: Q9EQ14 ELISA Kit: RayBio Mouse IL-23 alpha p19 ELISA	IL-28A (IFN-lambda 2) Gene Symbol: IFNL2 Accession #: Q4VK74 ELISA Kit: RayBio Mouse IL-28 ELISA	IL-3 Gene Symbol: IL3 Accession #: P01586 ELISA Kit: RayBio Mouse IL-3 ELISA
IL-4 Gene Symbol: IL4 Accession #: P07750 ELISA Kit: RayBio Mouse IL-4 ELISA	IL-5 Gene Symbol: IL5 Accession #: P04401 ELISA Kit: RayBio Mouse IL-5 ELISA	IL-6 Gene Symbol: IL6 Accession #: P08505 ELISA Kit: RayBio Mouse IL-6 ELISA	IL-7 Gene Symbol: IL7 Accession #: P10168 ELISA Kit: RayBio Mouse IL-7 ELISA	I-TAC (CXCL11) Gene Symbol: CXCL11 Accession #: Q9JHH5 ELISA Kit: RayBio Mouse I-TAC ELISA
KC (CXCL1) Gene Symbol: CXCL1 Accession #: P12850 ELISA Kit: RayBio Mouse KC ELISA	Leptin Gene Symbol: LEP Accession #: P41160 ELISA Kit: RayBio Mouse Leptin ELISA	LIX Gene Symbol: Cxcl5 Accession #: P50228 ELISA Kit: RayBio Mouse LIX ELISA	MCP-1 (CCL2) Gene Symbol: CCL2 Accession #: Q9QYD7 ELISA Kit: RayBio Mouse MCP-1 ELISA	MCP-5 Gene Symbol: Ccl12 Accession #: Q9QYD6 ELISA Kit: RayBio Mouse MCP-5 ELISA
M-CSF Gene Symbol: CSF1 Accession #: P07141 ELISA Kit: RayBio Mouse M-CSF ELISA	MDC (CCL22) Gene Symbol: CCL22 Accession #: O88430 ELISA Kit: RayBio Mouse MDC ELISA	MIG (CXCL9) Gene Symbol: CXCL9 Accession #: P18340 ELISA Kit: RayBio Mouse MIG ELISA	MIP-1 alpha (CCL3) Gene Symbol: CCL3 Accession #: P14096 ELISA Kit: RayBio Mouse MIP-1 alpha ELISA	MIP-1 gamma Gene Symbol: Ccl9 Accession #: Q5QNW2 ELISA Kit: RayBio Mouse MIP-1 gamma ELISA
MIP-2 Gene Symbol: Cxcl2 Accession #: P10889 ELISA Kit: RayBio Mouse MIP-2 ELISA	MIP-3 alpha (CCL20) Gene Symbol: CCL20 Accession #: O89093 ELISA Kit: RayBio Mouse MIP-3 alpha ELISA	Osteopontin (SPP1) Gene Symbol: SPP1 Accession #: P19008 ELISA Kit: RayBio Mouse OPN ELISA	Osteoprotegerin (TNFRSF11B) Gene Symbol: TNFRSF11B Accession #: O70202 ELISA Kit: RayBio Mouse OPG ELISA	Platelet Factor 4 (CXCL4) Gene Symbol: CXCL14 Accession #: Q9WUQ5 ELISA Kit: RayBio Mouse PF-4 ELISA
Prolactin Gene Symbol: PRL Accession #: P06879 ELISA Kit: RayBio Mouse Prolactin ELISA	Pro-MMP-9 Gene Symbol: Mmp9 Accession #: Q80XI8 ELISA Kit: RayBio Mouse pro-MMP-9 ELISA	P-Selectin Gene Symbol: SELP Accession #: Q01102 ELISA Kit: RayBio Mouse P-Selectin ELISA	RANTES (CCL5) Gene Symbol: CCL5 Accession #: P30882 ELISA Kit: RayBio Mouse RANTES ELISA	Resistin Gene Symbol: RETN Accession #: Q99P87 ELISA Kit: RayBio Mouse Resistin ELISA
SCF Gene Symbol: KITLG Accession #: P20826 ELISA Kit: RayBio Mouse SCF ELISA	SDF-1 alpha (CXCL12 alpha) Gene Symbol: CXCL12 Accession #: P40224 ELISA Kit: RayBio Mouse SDF-1 alpha ELISA	TARC (CCL17) Gene Symbol: CCL17 Accession #: Q9WUZ6 ELISA Kit: RayBio Mouse TARC ELISA	Thrombopoietin (TPO) Gene Symbol: THPO Accession #: P40226 ELISA Kit: RayBio Mouse TPO ELISA	TNF alpha Gene Symbol: TNF Accession #: Q62326 ELISA Kit: RayBio Mouse TNF-alpha ELISA
TNF RI (TNFRSF1A) Gene Symbol: TNFRSF1A Accession #: P25118 ELISA Kit: RayBio Mouse sTNFRI ELISA	TNF RII (TNFRSF1B) Gene Symbol: TNFRSF1B Accession #: O88734 ELISA Kit: RayBio Mouse sTNFRII ELISA	VCAM-1 (CD106) Gene Symbol: VCAM1 Accession #: P29533 ELISA Kit: RayBio Mouse VCAM-1 ELISA	VEGF-A Gene Symbol: VEGFA Accession #: Q00731 ELISA Kit: RayBio Mouse VEGF-A ELISA	VEGF-D Gene Symbol: FIGF Accession #: A2AIH3

Species Detected

Mouse

Application Notes

Suggested Application

Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range

Kit Components

Mouse Cytokine Array Q1000 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Lyophilized Standard Mix
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
Slide Washer/Dryer
Adhesive Plastic Strips
Manual

Other Materials Required

Distilled or deionized water
Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.