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Mouse Cytokine Array Q1000

Quantibody® Mouse Cytokine Array 1000 Kit. A combination of Mouse Cytokine Array Q4 and Mouse Cytokine Array Q5. Detects 80 Mouse Cytokines. Suitable for all liquid sample types.

Old price: $1,979.90
$1,583.92  
You save: $395.98 (20%)
QAM-CAA-1000-1
+

Antigen Information

Gene Symbols:
AXL | CCL1 | CCL11 | CCL12 | CCL17 | CCL2 | CCL20 | CCL22 | CCL24 | CCL3   View more

Assay Format

Number of Targets Detected:
80
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Other Body Fluids
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Product Features

  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 100 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
Amphiregulin
Axl
bFGF
BLC
(CXCL13)
CD27 Ligand
(TNFSF7)
CD30
(TNFRSF8)
CD30 Ligand
(TNFSF8)
CD40
(TNFRSF5)
CXCL16
EGF
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
E-Selectin
Fas Ligand
(TNFSF6)
Fractalkine
(CX3CL1)
GCSF
GITR
(TNFRSF18)
GM-CSF
HGF
I-309
(TCA-3/CCL1)
ICAM-1
(CD54)
IFN-gamma
IGF-1
IGFBP-2
IGFBP-3
IGFBP-5
IGFBP-6
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-1 ra
(IL-1 F3)
IL-10
IL-12 p40
  • Gene Symbol: IL12B
  • Accession #: P43432
IL-12 p70
  • Gene Symbol: IL12A
  • Accession #: P43431
IL-13
IL-15
IL-17A
IL-17E
(IL-25)
IL-17F
IL-2
IL-2 R alpha
IL-20
IL-21
IL-23 p19
IL-28A
(IFN-lambda 2)
IL-3
IL-4
IL-5
IL-6
IL-7
I-TAC
(CXCL11)
KC
(CXCL1)
Leptin
LIX
MCP-1
(CCL2)
MCP-5
M-CSF
MDC
(CCL22)
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
MIP-1 gamma
MIP-2
MIP-3 alpha
(CCL20)
Osteopontin
(SPP1)
Osteoprotegerin
(TNFRSF11B)
Platelet Factor 4
(CXCL4)
Prolactin
Pro-MMP-9
P-Selectin
RANTES
(CCL5)
Resistin
SCF
SDF-1 alpha
(CXCL12 alpha)
TARC
(CCL17)
Thrombopoietin
(TPO)
TNF alpha
TNF RI
(TNFRSF1A)
TNF RII
(TNFRSF1B)
VCAM-1
(CD106)
VEGF-A
VEGF-D
  • Gene Symbol: FIGF
  • Accession #: A2AIH3

Species Detected

Mouse

Application Notes

Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
  • Mouse Cytokine Array Q1000 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Slide Washer/Dryer
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Doan, Phuong L., et al. "Epidermal growth factor regulates hematopoietic regeneration after radiation injury." Nature medicine 19.3 (2013): 295-304.
    Species: Mouse
    Sample type: Bone Marrow Aspirate (BM was collected from adult, non-irradiated Tie2Cre;Bak1?/?;Baxfl/? mice and Tie2Cre;Bak1?/?;Baxfl/+ mice and C57Bl6 mice)
  2. Jurk D., Wilson C., Passos J., et al. Chronic inflammation induces telomere dysfunction and accelerates ageing in mice. Nature Communications 2, 2014, Article number: 4172. doi:10.1038/ncomms5172
    Species: Mouse
    Sample type: Conditioned Media (WT vs NFkb-/- mouse ear fibroblasts)
  3. Jurk D., Wilson C., Passos J., et al. Chronic inflammation induces telomere dysfunction and accelerates ageing in mice. Nature Communications 2, 2014, Article number: 4172. doi:10.1038/ncomms5172
    Species: Mouse
    Sample type: Tissue Lysate (Frozen Liver Lysates)
  4. Fritz J., et al. Depletion of tumor-associated macrophages slows the growth of chemically induced mouse lung adenocarcinomas. Front. Immunol., 25 November 2014 | doi: 10.3389/fimmu.2014.00587
    Species: Mouse
    Sample type: BAL Fluid
  5. Doan, Phuong L., et al. "Tie2+ bone marrow endothelial cells regulate hematopoietic stem cell regeneration following radiation injury." Stem cells31.2 (2013): 327-337.
    Species: Mouse
    Sample type: Bone Marrow Aspirate (mice bearing deletion of the pro-apoptotic proteins, BAK and BAX, Tie2)
  6. Correnti JM., Cook D., Aksamitiene E., et al. Adiponectin fine-tuning of liver regeneration dynamics revealed through cellular network modeling. J Physiol. 2014 Nov 10. [Epub ahead of print]. doi: 10.1113/jphysiol.2014.284109.
    Species: Mouse
    Sample type: Tissue Lysate (Liver Lysate)
  7. Correnti JM., Cook D., Aksamitiene E., et al. Adiponectin fine-tuning of liver regeneration dynamics revealed through cellular network modeling. J Physiol. 2014 Nov 10. [Epub ahead of print]. doi: 10.1113/jphysiol.2014.284109.
    Species: Mouse
    Sample type: Serum
  8. De Pascalis R., Mittereder L., Kennett N., Elkins K. Activities of Murine Peripheral Blood Lymphocytes Provide Immune Correlates That Predict Francisella tularensis Vaccine Efficacy. Infect Immun. 2016 Mar 24;84(4):1054-61. doi: 10.1128/IAI.01348-15.
    Species: Mouse
    Sample type: Conditioned Media (Francisella infected bone marrow macrophages)
  9. Trune, Dennis R., et al. "Simultaneous measurement of multiple ear proteins with multiplex ELISA assays." Hearing research 275.1 (2011): 1-7.
    Species: Mouse
    Sample type: Tissue lysate
  10. Chen, D., Wang, R., Long, M., Li, W., Xiao, B., Deng, H. ... Hao, W. (2021). Identification of in vitro and in vivo oncolytic effect in colorectal cancer cells by Orf virus strain NA1/11. Oncology Reports, 45, 535-546.
    Species: Mouse
    Sample type: Conditioned Media (cell culture media)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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