RayBio® Mouse VEGF-D ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Estimated Lead Time | 1-2 business days |
| Species | Mouse |
| Sensitivity | 0.07 ng/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 0.07 ng/ml - 20 ng/ml |
| Specificity | This ELISA kit shows no cross-reactivity with the following cytokines tested: mouse Amphiregulin, Axl, CD27 Ligand (TNFSF7), CD30 (TNFRSF8), CD40 (TNFRSF5), CXCL16, EGF, E-Selectin, Fractalkine (CX3CL1), GITR (TNFRSF18), HGF, IGF-1, IGFBP-2, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 ra (IL-1 F3), IL12 p70, IL-17E (IL-25), IL-17F, IL-2 R alpha, IL-20, IL-23 p19, IL-28A (IFN-lambda 2), I-TAC (CXCL11), MDC (CCL22), MIP2, MIP-3 alpha (CCL20), Osteopontin (SPP1), Osteoprotegerin (TNFRSF11B), Prolactin, Pro-MMP-9, PSelectin, Resistin, SCF, SDF-1 alpha (CXCL12 alpha), Thrombopoietin (TPO), VCAM-1 (CD106), VEGF-A. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | P97946 |
| Gene Symbols | Vegfd |
| Protein Name / Synonyms | Vascular endothelial growth factor D (VEGF-D) (c-Fos-induced growth factor) (FIGF) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis, Vascular Biology |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
| Sample Type | Average % Recovery | Range (%) |
|---|---|---|
| Serum | 99.55 | 73-129 |
| Plasma | 122.6 | 109-136 |
| Cell culture media | 111.0 | 96-121 |
Linearity Results
| Sample Type | Serum | Plasma | Cell Culture Media | |
|---|---|---|---|---|
| 1:2 | Average % of Expected | 118.7 | 124.1 | 136.9 |
| Range (%) | 111-127 | 123-125 | 136-138 | |
| 1:4 | Average % of Expected | 125.0 | 127.3 | 126.1 |
| Range (%) | 123-127 | 111-137 | 122-130 | |
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
