Serine/threonine-protein phosphatase PP1-alpha catalytic subunit, PP-1A, PPP1CA, PPP1A, PP1alpha.

MGSSHHHHHH SSGLVPRGSH MSDSEKLNLD SIIGRLLEVQ GSRPGKNVQL TENEIRGLCL KSREIFLSQP ILLELEAPLK ICGDIHGQYY DLLRLFEYGG FPPESNYLFL GDYVDRGKQS LETICLLLAY KIKYPENFFL LRGNHECASI NRIYGFYDEC KRRYNIKLWK TFTDCFNCLP IAAIVDEKIF CCHGGLSPDL QSMEQIRRIM RPTDVPDQGL LCDLLWSDPD KDVQGWGEND RGVSFTFGAE VVAKFLHKHD LDLICRAHQV VEDGYEFFAK RQLVTLFSAP NYCGEFDNAG AMMSVDETLM CSFQILKPAD KNKGKYGQFS GLNPGGRPIT PPRNSAKAKK.

Greater than 80.0% as determined by SDS-PAGE.

Sterile filtered yellowish solution.

PPP1CA protein solution (0.2mg/ml) containing 50mM Tris-HCl (pH8.5),0.2M NaCl, 1mM DTT, 0.1mM PMSF, 1mM MnCl2 and 50% glycerol. ,

Specific activity: >,3,000 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10mM of substrate.

Description

PPP1CA Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 350 amino acids (1-330 a.a) and having a molecular mass of 39.7kDa.PPP1CA is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Stability & Storage

Storage/Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Serine/threonine-protein phosphatase PP1-alpha catalytic subunit, PP-1A, PPP1CA, PPP1A, PP1alpha.

Greater than 80.0% as determined by SDS-PAGE.

Sterile filtered yellowish solution.

PPP1CA protein solution (0.2mg/ml) containing 50mM Tris-HCl (pH8.5),0.2M NaCl, 1mM DTT, 0.1mM PMSF, 1mM MnCl2 and 50% glycerol. ,

Description

Stability & Storage

Storage/Stability

Size	5 µg, 50 µg
Estimated Lead Time	2-3 weeks
Species	Human
Accession Number	P62136
Gene Id	5499
Protein Name / Synonyms	Serine/threonine-protein phosphatase PP1-alpha catalytic subunit, PP-1A, PPP1CA, PPP1A, PP1alpha.
Expressed Region	MGSSHHHHHH SSGLVPRGSH MSDSEKLNLD SIIGRLLEVQ GSRPGKNVQL TENEIRGLCL KSREIFLSQP ILLELEAPLK ICGDIHGQYY DLLRLFEYGG FPPESNYLFL GDYVDRGKQS LETICLLLAY KIKYPENFFL LRGNHECASI NRIYGFYDEC KRRYNIKLWK TFTDCFNCLP IAAIVDEKIF CCHGGLSPDL QSMEQIRRIM RPTDVPDQGL LCDLLWSDPD KDVQGWGEND RGVSFTFGAE VVAKFLHKHD LDLICRAHQV VEDGYEFFAK RQLVTLFSAP NYCGEFDNAG AMMSVDETLM CSFQILKPAD KNKGKYGQFS GLNPGGRPIT PPRNSAKAKK.
Expression System	E. coli
Purity	Greater than 80.0% as determined by SDS-PAGE.
Format	Sterile filtered yellowish solution.
Formulation	PPP1CA protein solution (0.2mg/ml) containing 50mM Tris-HCl (pH8.5),0.2M NaCl, 1mM DTT, 0.1mM PMSF, 1mM MnCl2 and 50% glycerol. ,
Activity	Specific activity: >,3,000 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10mM of substrate.
Shipping Type	Blue ice
Storage	-20°C

Size	5 µg, 50 µg
Estimated Lead Time	2-3 weeks
Species	Human
Accession Number	P62136
Gene Id	5499
Protein Name / Synonyms	Serine/threonine-protein phosphatase PP1-alpha catalytic subunit, PP-1A, PPP1CA, PPP1A, PP1alpha.
Expressed Region	MGSSHHHHHH SSGLVPRGSH MSDSEKLNLD SIIGRLLEVQ GSRPGKNVQL TENEIRGLCL KSREIFLSQP ILLELEAPLK ICGDIHGQYY DLLRLFEYGG FPPESNYLFL GDYVDRGKQS LETICLLLAY KIKYPENFFL LRGNHECASI NRIYGFYDEC KRRYNIKLWK TFTDCFNCLP IAAIVDEKIF CCHGGLSPDL QSMEQIRRIM RPTDVPDQGL LCDLLWSDPD KDVQGWGEND RGVSFTFGAE VVAKFLHKHD LDLICRAHQV VEDGYEFFAK RQLVTLFSAP NYCGEFDNAG AMMSVDETLM CSFQILKPAD KNKGKYGQFS GLNPGGRPIT PPRNSAKAKK.
Expression System	E. coli
Purity	Greater than 80.0% as determined by SDS-PAGE.
Format	Sterile filtered yellowish solution.
Formulation	PPP1CA protein solution (0.2mg/ml) containing 50mM Tris-HCl (pH8.5),0.2M NaCl, 1mM DTT, 0.1mM PMSF, 1mM MnCl2 and 50% glycerol. ,
Activity	Specific activity: >,3,000 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10mM of substrate.
Shipping Type	Blue ice
Storage	-20°C

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