Human Cytokine Array Q1000

Quantibody® Human Cytokine Array 1000 Kit. A combination of Human Inflammation Array Q3 and Human Growth Factor Array Q1. Detects 80 Human Inflammatory and Growth Factors. Suitable for all liquid sample types.

QAH-CAA-1000-1
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Antigen Information

Gene Symbols:
AREG | BDNF | BMP4 | BMP5 | BMP7 | CCL1 | CCL11 | CCL15 | CCL2 | CCL24   View more

Assay Format

Number of Targets Detected:
80
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Other Body Fluids
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Product Features

  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 100 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
Amphiregulin
BDNF
beta-NGF
bFGF
BLC
(CXCL13)
BMP-4
BMP-5
BMP-7
EGF
EGFR
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
FGF-4
FGF-7
(KGF)
GCSF
GDF-15
GDNF
GM-CSF
Growth Hormone
EG-VEGF
(PK1)
HB-EGF
HGF
I-309
(TCA-3/CCL1)
ICAM-1
(CD54)
IFN-gamma
IGF-1
IGFBP-1
IGFBP-2
IGFBP-3
IGFBP-4
IGFBP-6
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-1 ra
(IL-1 F3)
IL-10
IL-11
IL-12 p40
IL-12 p70
IL-13
IL-15
IL-16
IL-17A
IL-2
IL-4
IL-5
IL-6
IL-6 R
IL-7
IL-8
(CXCL8)
Insulin
MCP-1
(CCL2)
M-CSF
M-CSF R
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
MIP-1 beta
(CCL4)
MIP-1 delta
(CCL15)
NGFR
(TNFRSF16)
NT-3
NT-4
Osteoprotegerin
(TNFRSF11B)
PDGF-AA
PDGF-BB
PLGF
RANTES
(CCL5)
SCF
SCF R
(CD117/c-kit)
TGF alpha
TGF beta 1
TGF beta 3
  • Gene Symbol: TGFB3
  • Gene ID: 7043
  • Accession #: P10600
TIMP-1
TIMP-2
TNF alpha
TNF beta
(TNFSF1B)
TNF RI
(TNFRSF1A)
TNF RII
(TNFRSF1B)
VEGF-A
VEGF-D
VEGFR2
VEGFR3

Species Detected

Human

Application Notes

Suggested Application
  • Multiplexed Protein Detection
  • Biomarker Screening
  • Identifying Key Factors
  • Confirming Biological Process
  • Biomarker Validation
  • Validation of Antibody Array Results
  • Quantitative Protein Detection
  • Establishing Normal Range
Kit Components
  • Human Cytokine Array Q1000 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Slide Washer/Dryer
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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Q-Analyzer Bundle Discount

70% off the Q-Analyzer tool when you purchase the array and tool together!


Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department (orders@raybiotech.com).

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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