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Human Th1/Th2/Th17 Array Q1

$721.00
QAH-TH17-1-1

Quantibody® Human Th17 Array 1 Kit. Detects 20 Human Th1, Th2, and Th17 Cytokines. Suitable for all liquid sample types.

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5% Off When Ordering Online

Product Features

  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information

GM-CSF
IFN-gamma
IL-1 beta
(IL-1 F2)
IL-10
IL-12 p70
IL-13
IL-17A
IL-17F
IL-2
IL-21
IL-22
IL-23
IL-28A
(IFN-lambda 2)
IL-4
IL-5
IL-6
MIP-3 alpha
(CCL20)
TGF beta 1
TNF alpha
TNF beta
(TNFSF1B)

Species Detected

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
  • Human Th1/Th2/Th17 Array Q1 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • 30 ml Centrifuge Tube
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Number of Targets Detected

20

Antigen Information

Gene Symbol:
  • CCL20
  • CSF2
  • IFNG
  • IFNL2
  • IL10
  • IL12A
  • IL13
  • IL17A
  • IL17F
  • IL1B
  • IL2
  • IL21
  • IL22
  • IL23A
  • IL4
  • IL5
  • IL6
  • LTA
  • TGFB1
  • TNF

Product Specifications

Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Array/ELISA Format

Number of Targets Detected:
20
Species Detected:
  • Human
Solid Support:
Glass Slide
Design Principle:
  • Sandwich-based
Quantitative/Semi-Quantitative:
Quantitative
Method of Detection:
Fluorescence Laser Scanner
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants
  • Cell Lysates
  • Tissue Lysates
  • Other Body Fluids

  1. Canete J., Celis R., Yeremenko N., et al. Ectopic lymphoid neogenesis is strongly associated with activation of the IL-23 pathway in rheumatoid synovitis. Arthritis Res Ther. 2015 Jul 9;17:173. doi: 10.1186/s13075-015-0688-0.
    Species: Human
    Sample Type: Synovial Fluid

  2. Gomez-Puerta J., et al. Differences in synovial fluid cytokine levels but not in synovial tissue cell infiltrate between anti-citrullinated peptide/protein antibody-positive and -negative rheumatoid arthritis patients. Gómez-Puerta et al. Arthritis Research & Therapy 2013, 15:R182
    Species: Human
    Sample Type: Synovial Fluid

  3. Kotlarz D, Ziętara N, Uzel G, et al. Loss-of-function mutations in the IL-21 receptor gene cause a primary immunodeficiency syndrome. J. Exp. Med. 2013 Mar 11;210(3):433-443.
    Species: Human
    Sample Type: Conditioned Media

  4. Yang P., Foster CS. Interleukin 21, interleukin 23, and transforming growth factor β1 in HLA-A29-associated birdshot retinochoroidopathy. Am J Ophthalmol. 2013 Aug;156(2):400-406.e2. doi: 10.1016/j.ajo.2013.03.004.
    Species: Human
    Sample Type: Serum

  5. Liu R, Wu Q, Su D, et al. A regulatory effect of IL-21 on T follicular helper-like cell and B cell in rheumatoid arthritis. Arthritis Research & Therapy. 2012;14(6):R255. doi:10.1186/ar4100.
    Species: Human
    Sample Type: Serum

  6. Celis R., et al. Synovial cytokine expression in psoriatic arthritis and associations with lymphoid neogenesis and clinical features. Celis et al. Arthritis Research & Therapy 2012, 14:R93
    Species: Human
    Sample Type: Synovial Fluid

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Q-Analyzer Bundle Discount

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1 x $721.00
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70% off the Q-Analyzer tool when you purchase the array and tool together!


Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department (orders@raybiotech.com).
Total list price $1,044.00
Price for all $817.90
U.S. International

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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