Rabbit Anti-Human MMP7 Antibody
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Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P09237 |
| Gene Id | 4316 |
| Gene Symbols | MMP7 MPSL1 PUMP1 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was a partial length of recombinant protein of human matrix metalloproteinase 7. Thisantibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purifiedfrom rabbit serum by ammonium sulphate precipitation, and followed by Protein A/G affinitychromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1:10, 000(at least detecting 100ng/ml)), Western Blotting (recommended work dilution= 1:1000-5000(at least)), Immunodetection Analysis: Representative blot from a previous lot. Lane 1.recombinant proteinMMP7. The membrane blot was probed with anti-MMP7 primary antibody(0.25?g/ml). Proteins werevisualized using a goat anti-rabbit secondary antibodyconjugated to HRP and chemiluminescence detectionsystem. Arrows indicate cellular MMP7 from humanand mouse cells (35 kDa). |
| Antigen Catalog Number | RB-15-0007P |
| Reconstitution | Supplied as lyophilized and purified antibody originally containing PBS, without Preservative Stabilizers,liking Sodium Azide. The final concentration is indicated on the shipping vial. Please avoid freeze-thaw cycles. The antibody is stable for at least years from the data of receipt when stored at -20°C to -70°C. Reconstituted antibody (recommended with H20 1 mg/ml) can also be aliquoted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss activity. Upon reconstitution, the antibody can be stored at 40°C for 1 month. |
| Research Area | Cardiovascular Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Matrix metalloproteinase 7, play an important role in the early steps of tumor progression with amolecular weight of 37.73 kDa, regulator of matrix remodeling, component of MMP cluster, matrilysin,stromelysin subclass of MMP, expressed in uterus, also expressed in Paneth cells (in mice), colocalizingand regulating DEFA5, generating angiostatin fragments by hydrolyzing plasminogen, overexpressed in82% of colorectal, regulating by beta catenin/CF4 and important factor in early tumor growthprogression and metastasis.
Specificity
The rabbit anti-matrix metalloproteinase 7 antibody specifically detects target derived from humanrecombinant protein as well as selected cell lysates. Cross reactivity with mouse and rat MMP7 was nottested yet.
Storage
Store at -20°C, if not intended for use within a month, keep it at 4°C and minimize freezing and thawing when use.
Related Products
Human MMP-7 ELISA Kit available on For Cell Culture Supernatant (Cat# ELH-MMP7-001 ); Matrix metalloproteinase 7 (matrilysin, uterine) (Cat#RB-15-0007P-3 )
References
- Ivan O Rosas,et al., (2008) MMP1 and MMP7 as potential peripheral blood biomarkers in idiopathicpulmonary fibrosis. PLoS Med.5(4): e93;
- Tolboom, TC,et al. (2002) Invasive properties of fibroblast-like synoviocytes: correlation with growthcharacteristics and expression of MMP-7, MMP-3, and MMP-70. Annals of the Rheumatic Diseases61(11): 975-980.
Expiration:
12 months from the date of shipment when stored properly.
