Rabbit anti-MMP7 Biotinylated Antibody, 50 ug
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Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P09237 |
| Gene Id | 4316 |
| Gene Symbols | MMP7 MPSL1 PUMP1 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was a partial length of recombinant protein of human matrix metalloproteinase 7. Thisantibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purifiedfrom rabbit serum by ammonium sulphate precipitation, and followed by Protein A/G affinitychromatography. |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1:10, 000(at least detecting 100 µg/ml)), Western Blotting (recommended work dilution= 1:1000-5000(at least)), Immunodetection Analysis: Representative blot from a previous lot. Lane 1.recombinant proteinMMP7. The membrane blot was probed with anti-MMP7 primary antibody(0.25 µg/ml). Proteins werevisualized using a goat anti-rabbit secondary antibodyconjugated to HRP and chemiluminescence detectionsystem. Arrows indicate cellular MMP7 from humanand mouse cells (35 kDa). |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Research Area | Cardiovascular Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Matrix metalloproteinase 7, play an important role in the early steps of tumor progression with amolecular weight of 37.73 kDa, regulator of matrix remodeling, component of MMP cluster, matrilysin,stromelysin subclass of MMP, expressed in uterus, also expressed in Paneth cells (in mice), colocalizingand regulating DEFA5, generating angiostatin fragments by hydrolyzing plasminogen, overexpressed in82% of colorectal, regulating by beta catenin/CF4 and important factor in early tumor growthprogression and metastasis.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
The rabbit anti-matrix metalloproteinase 7 antibody specifically detects target derived from humanrecombinant protein as well as selected cell lysates. Cross reactivity with mouse and rat MMP7 was nottested yet.
Related Products
Human MMP-7 ELISA Kit available on For Cell Culture Supernatant (Cat# ELH-MMP7-001 ); Matrix metalloproteinase 7 (matrilysin, uterine) (Cat#RB-15-0007P-3 )
References
- Ivan O Rosas,et al., (2008) MMP1 and MMP7 as potential peripheral blood biomarkers in idiopathicpulmonary fibrosis. PLoS Med.5(4): e93;
- Tolboom, TC,et al. (2002) Invasive properties of fibroblast-like synoviocytes: correlation with growthcharacteristics and expression of MMP-7, MMP-3, and MMP-70. Annals of the Rheumatic Diseases61(11): 975-980.
Expiration:
12 months from the date of shipment when stored properly.
