Rabbit Anti-Relish Antibody
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Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Insect |
| Accession Number | NP_477094 |
| Clonality | Polyclonal |
| Preparation | This antibody was produced from a rabbit immunized with the KLH-conjugated peptide derived from Drosophila Relish. The IgG fraction of immunized serum was purified by affinity chromatography. |
| Immunogen Sequence | CQLGATNLINSTGVSFG |
| Recommended Applications | ELISA (recommended work dilution= 1:1000-5000), Western Blotting, Immunostaining(recommended work dilution= 1:50), (see image) Nuclear translocation of Relish after bacterial challenge: Third instar larva were parenterally challenged with Erwinia carotovora (a natural fly pathogen). After 2 hours, the larval fat body was dissected, fixed with paraformaldehyde, and immunostained with RB-14-0004 (1/50 dilution), followed by secondary incubation with Goat anti-rabbit-Cy5. Tissues were also stained with SYTO24 DNA stain. Images captured by confocal microscopy |
| Reconstitution | Supplied as 100 µg of purified, lyophilized antibody. Reconstitute with sterile water to 1 mg/ml, then aliquot and store at 4°C for 1 month. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
The NF-kB-like transcription factor Relish plays a central role in the innate immune response of Drosophila. Relish is a bipartite NF-kB precursor protein, with an N-terminal Rel homology domain and a C-terminal IkappaB-like domain, similar to mammalian p100 and p105. Unlike mammalian NF-kB proteins, Relish is activated by endoproteolytic cleavage to generate a DNA-binding Rel homology domain (545 a.a.) and a stable IkB-like fragment (426 a.a.).
Specificity
The antibody detects specifically target peptide derived from Drosophila Relish by ELISA and Dot Blotting. Also detects cleaved and uncleaved Relish in PFA-fixed Drosphila tissue (see image). Cross reactivity with human, mouse and rat have not been tested.
Storage
The antibody is stable for at least 1 year from the date of shipment when stored at -20°C to -70°C. Please avoid freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.
