Rat Fractalkine ELISA

RayBio® Rat Fractalkine (CX3CL1) ELISA Kit for cell culture supernatants, plasma, and serum samples.

ELR-Fractalkine-1
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Antigen Information

Accession Number:
  • Q6IRF7
Gene Symbols:
  • CX3CL1
  • RCG_39059
Protein Name & Synonyms:
Chemokine (C-X3-C motif) ligand 1 (Cx3cl1 protein) (Fractalkine)
Target Species:
  • Rat

Assay Format

Detection Range:
5 pg/ml - 2000 pg/ml
Recommended Dilution (serum & plasma):
3 fold
Sensitivity:
5 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: rat CINC-2, CINC-3, CNTF, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta-NGF, TIMP-1, TNF-alpha, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Rat
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Koyama Y, Kotani M, Sawamura T, et al. Different actions of endothelin-1 on chemokine production in rat cultured astrocytes: reduction of CX3CL1/fractalkine and an increase in CCL2/MCP-1 and CXCL1/CINC-1. J Neuroinflamm. 2013;10:51.
    Species: Rat
    Sample type: Conditioned Media
  2. Uchida, Masahiko, et al. "ERK pathway and sheddases play an essential role in ethanol-induced CX3CL1 release in pancreatic stellate cells." Laboratory Investigation 93.1 (2013): 41-53.
    Species: Rat
    Sample type: Conditioned Media
  3. Huang, Libin, Wei Xu, and Guoxing Xu. "Transplantation of CX3CL1-expressing mesenchymal stem cells provides neuroprotective and immunomodulatory effects in a rat model of retinal degeneration." Ocular immunology and inflammation21.4 (2013): 276-285.
    Species: Rat
    Sample type: Conditioned Media
  4. Gunay, Y., et al. "A Novel Mechanism of Anti–T-Lymphocyte Globulin Mediated by Fractalkine in Renal Ischemia–Reperfusion Injury in Rats." Transplantation proceedings. Vol. 45. No. 6. Elsevier, 2013.
    Species: Rat
    Sample type: Plasma
  5. Bachstetter AD., et al. Fractalkine and CX3CR1 regulate hippocampal neurogenesis in adult and aged rats. Neurobiol Aging. 2011 Nov;32(11):2030-44. doi: 10.1016/j.neurobiolaging.2009.11.022.
    Species: Rat
    Sample type:
  6. Bachstetter AD, Morganti JM, Jernberg J, et al. Fractalkine and CX3CR1 regulate hippocampal neurogenesis in adult and aged rats. Neurobiology of aging. 2011;32(11):2030-2044. doi:10.1016/j.neurobiolaging.2009.11.022.
    Species: Rat
    Sample type: Tissue lysate
  7. Bitar, Milad S., and Fahd Al-Mulla. "A defect in Nrf2 signaling constitutes a mechanism for cellular stress hypersensitivity in a genetic rat model of type 2 diabetes." American Journal of Physiology-Endocrinology and Metabolism 301.6 (2011): E1119-E1129.
    Species: Rat
    Sample type: Conditioned Media
  8. Clark AK, Yip P, Grist J, Gentry C, Staniland A. Inhibition of spinal microglial cathepsin S for the reversal of neuropathic pain. Proc Natl Acad Sci USA. 2007;104(25):10655-10660.
    Species: Rat
    Sample type:
  9. Clark AK., et al. Inhibition of spinal microglial cathepsin S for the reversal of neuropathic pain. Proc Natl Acad Sci U S A. 2007 Jun 19;104(25):10655-60.
    Species: Rat
    Sample type:

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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