• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

• Pre-Coated 96-well Strip Microplate
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• Wash Buffer
• TMB One-Step Substrate Reagent
• Stop Solution
• Assay Diluent(s)
• Adhesive Sealing Film

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 µL to 1 µL volumes
• Adjustable 1-25 µL pipettes for reagent preparation
• 100 µL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Log-log graph paper or computer and software for ELISA data analysis
• Tubes to prepare standard or sample dilutions
• Plastic wrap

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µL standard or sample to each well. Incubate 2.5 hours at room temperature.
3. Add 100 µL prepared biotin antibody to each well. Incubate 1 hour at room temperature.
4. Add 100 µL prepared Streptavidin solution to each well. Incubate 45 minutes at room temperature.
5. Add 100 µL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 µL Stop Solution to each well. Read at 450 nm immediately

We manufacture our ELISAs in-house, so we can build a custom version for your study.

• Post-translational modifications (PTMs): Detect modified RANK (TNFRSF11A), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
• Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
• Also available: More sensitive platforms such as Simoa^® and NexaTag^®, multiplex panels, bulk lots, and other species when suitable reagents exist

Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.

Learn more about custom ELISA development