RayPlex is a multiplexed sandwich- and bead-based quantitative antibody array for the simultaneous detection of multiple proteins using small sample volumes. It couples the versatility of RayBiotech's vast antibody pair library with familiar, reliable flow cytometry methodology. Together this creates a multiplex bead- based array requiring no dedicated instrumentation, only common flow cytometers equipped with blue and red lasers. This kit can also be used with Luminex systems.

RayPlex®-MagPro kits use magnetic beads. If preferred, an automatic plate washing system with magnetic separation ability can be used for washing steps. For data in this manual, a BioTek 405 TS Washer and LifeSep™ 96F magnetic separator unit were used.

Alzheimer’s Disease is a neurodegenerative disease affecting one’s memory and cognitive function. The accumulation of beta-amyloid and phosphorylated Tau has been identified as having a strong correlation to the disease. This kit tests for two forms of phosphorylated Tau: pTau217 and pTau181. Both forms are associated with the early stages of disease. More research is necessary in order to confirm the mechanism that links these biomarkers and Alzheimer’s Disease, though, many researchers include the targets in their study of the disease and its progression.

• Less sample, more data: up to 2 proteins can be quantified from only 25 µl or less of your precious sample
• 4 hour processing time
• 5-10x more cost-effective than ELISA
• Compatible with most flow cytometers
• No specialized or dedicated instrument(s) needed
• High specificity and system reproducibility
• Well-suited for high throughput assays

• RayPlex^® MagPro Multiplex Bead Cocktail
• Assay Diluent
• Wash Buffer
• RayBio^® Serum/Plasma Diluent
• Lyophilized Protein Standard Mix
• Biotinylated Detection Antibody Cocktail
• PE Conjugate
• Flow Cytometer Setup Bead Cocktail
• Flat-bottom 96-well Microplate
• V-Bottom 96-well Microplate

• Orbital 96-well plate shaker (with ability to reach 1000 rpm)
• Flow Cytometer with blue (or green) and red lasers capable of measuring phycoerythrin (PE), Alexa Fluor 700 (AF700), and Alexa Fluor 647 (AF647)/APC channels OR Luminex instrument
• Aluminum foil or 96-well Plate Aluminum Sealers
• Distilled water
• PBS
• 1.5 ml polypropylene microcentrifuge tubes or similar
• Microcentrifuge
• Recommended: Automated plate washing system with magnetic separation
• Optional: 96-well round bottom plate (to prepare samples)
• Optional: High throughput sampler (HTS) for 96-well plate reading
• Optional: Vacuum manifold compatible with a 96-well microplate if using a Filter 96-Well Microplate

Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex™ bead arrays. Get a quote!

1. Prepare Reagents, Standards, and Samples
2. Add RayPlex™ Multiplex Bead Cocktail to each well
3. Incubate with Samples and Standards
4. Incubate with Biotinylated Detection Antibody Cocktail
5. Incubate with Streptavidin-Conjugated Fluorophore
6. Analyze with a compatible flow cytometer
7. Perform data analysis

Upon receipt, the Lyophilized Protein Standard Mix should be stored at -20°C or below. Other components should be stored at 4°C. Use reagents immediately after preparation. Additional vials of the Lyophilized Protein Standard Mix can be purchased separately. If stored at the proper temperature, the kit will retain complete activity for at least 6 months.