Product Features
- Less sample, more data: up to 2 proteins can be quantified from only 25 µL or less of your precious sample
- 4 hour processing time
- 5-10x more cost-effective than ELISA
- Compatible with most flow cytometers
- No specialized or dedicated instrument(s) needed
- High specificity and system reproducibility
- Well-suited for high throughput assays
Target Names
pTau217 and pTau181
Kit Components
- RayPlex® Multiplex Bead Cocktail
- Assay Diluent
- Wash Buffer
- RayBio® Human Serum/Plasma Diluent
- Lyophilized Protein Standard Mix
- Biotinylated Detection Antibody Cocktail
- PE Conjugate
- Flow Cytometer Setup Bead Cocktail
- V-bottom 96-well Microplate
- Filter 96-well Microplate
Additional Materials Required
- Orbital 96-well plate shaker (with ability to reach 1000 rpm)
- Flow cytometer with blue (or green) and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (APC) channels
- Aluminum foil or 96-well Plate Aluminum Sealers
- Distilled water
- PBS
- 1.5 mL polypropylene microcentrifuge tubes or similar
- Microcentrifuge
- Recommended: Vacuum manifold compatible with a 96-well microplate if using the Filter 96-Well Microplate
- Optional: 96-well round bottom plate (to prepare samples)
- Optional: High throughput sampler (HTS) for 96-well plate reading
Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex™ bead arrays. Get a quote!
Assay Procedure Summary
- Prepare Reagents, Standards, and Samples
- Add RayPlex® Multiplex Bead Cocktail to each well
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluorophore
- Analyze with a compatible flow cytometer
- Perform data analysis
Storage/Stability
Upon receipt, the Lyophilized Protein Standard Mix should be stored at -20°C or below. Other components should be stored at 4°C. Use reagents immediately after preparation. Additional vials of the Lyophilized Protein Standard Mix can be purchased separately. If stored at the proper temperature, the kit will retain complete activity for at least 6 months.
