Product Features
- Less sample, more data: up to 70 proteins can be quantified from only 25 µL or less of your precious sample
- 4 hour processing time
- 5-10x more cost-effective than ELISA
- Compatible with most flow cytometers
- No specialized or dedicated instrument(s) needed
- High specificity and system reproducibility
- Well-suited for high throughput assays
Target Names
Target Protein Names, Accession Numbers, and IDs [Excel Download]
| Activin A (INHBA) | AgRP | APRIL (TNFSF13) | BAFF (TNFSF13B) | BCAM (CD239) |
| BLC (BCA-1 / CXCL13) | CD40 (TNFRSF5) | CTACK (CCL27) | CXCL1 (GRO alpha / KC / CINC-1) | CXCL16 (SR-PSOX) |
| Eotaxin-2 (MPIF-2 / CCL24) | Fas (Apo-1 antigen / TNFRSF6 / CD95) | Fas Ligand (TNFSF6 / CD178 / FASLG) | Fc gamma RIIB (CD32 / FCGR2B) | Fractalkine (CX3CL1) |
| GCSF (CSF3) | GM-CSF (CSF2) | I-309 (TCA-3 / CCL1) | ICAM-1 (CD54 / ICAM1) | IFN-alpha (IFNA1) |
| IFN-beta (IFNB1) | IFN-gamma (IFNG) | IL-1 beta (IL-1F2 / IL1B) | IL-10 (IL10) | IL-12 p70 (IL12A) |
| IL-13 (IL13) | IL-15 (IL15) | IL-16 (CXCR4 / IL16) | IL-17A (IL17A) | IL-1R-2 (IL-1RT-2 / IL1R2) |
| IL-2 (IL2) | IL-23 alpha (IL23A) | IL-3 (IL3) | IL-34 (IL34) | IL-4 (IL4) |
| IL-5 (IL5) | IL-6 (IL6) | IL-6R (IL6RA) | IL-9 (IL9) | JAM-A (CD321 / F11R) |
| Leptin (Lenep) | Lungkine (CXCL15) | Lymphotactin (XCL1) | M-CSF (CSF1) | MCP-1 (CCL2) |
| MCP-3 (MARC / CCL7) | MDC (CCL22) | MIP-1 alpha (CCL3) | MIP-1 beta (CCL4) | MIP-2 alpha (CXCL2) |
| MIP-3 alpha (CCL20) | MIP-3 beta (CCL19) | Oncostatin M (OSM) | PDGFB | RANTES (CCL5) |
| SCF (KITLG) | SDF-1 alpha (CXCL12 alpha) | Siglec-12 (SIGLEC12) | Sonic Hedgehog N-Terminal (Shh-N) | TARC (CCL17 / ABCD-2) |
| TGF beta 1 (TGFB1) | TGF beta 2 (TGFB2) | TIMP-4 (TIMP4) | TNF alpha (TNFA) | TNF R1 (TNFRSF1A) |
| TNF R2 (TNFRSF1B) | TRAIL (TNFSF10) | TREM-1 (TREM1) | VCC-1 (CXCL17) | VEGFA |
Kit Components
- RayPlex® Magnetic Multiplex Bead Cocktail
- PBS
- Assay Diluent
- Wash Buffer
- RayBio® Serum/Plasma Diluent
- Lyophilized Protein Standard Mix
- Biotinylated Detection Antibody Cocktail
- PE Conjugate
- Flow Cytometer Setup Bead Cocktail
- V-bottom 96-well Microplate
- Flat-bottom 96-well Microplate
Additional Materials Required
- Orbital 96-well plate shaker (with ability to reach 1000 rpm)
- Flow Cytometer with blue (or green) and red lasers capable of measuring phycoerythrin (PE), Alexa Fluor 700 (AF700), and Alexa Fluor 647 (AF647)/APC channels OR Luminex instrument
- Aluminum foil or 96-well Plate Aluminum Sealers
- Deionized (DI) water
- 1.5 mL polypropylene microcentrifuge tubes or similar
- Microcentrifuge
- Recommended: Automated plate washing system with magnetic separation
- Optional: 96-well round bottom plate (to prepare samples)
- Optional: High throughput sampler (HTS) for 96-well plate reading
- Optional: Vacuum manifold compatible with a 96-well microplate if using a Filter 96-Well Microplate
Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex™ bead arrays. Get a quote!
Assay Procedure Summary
- Prepare Reagents, Standards, and Samples
- Add RayPlex® Magnetic Multiplex Bead Cocktail to each well
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with PE Conjugate
- Analyze with a compatible flow cytometer
- Perform data analysis
Storage/Stability
Upon receipt, the Lyophilized Protein Standard Mix should be stored at -20°C or below. Other components should be stored at 4°C. Use reagents immediately after preparation. If stored at the proper temperature, the kit will retain complete activity for at least 6 months.