Recombinant Human HGF receptor (Hepatocyte growth factor receptor), N-terminal extracellular domain, derived from the transfected human HEK293 cells
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Product Description
Specifications
| Size | 10 µg, 50 µg, 100 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P08581 |
| Gene Id | 4233 |
| Gene Symbols | MET |
| Protein Name / Synonyms | Met Proto-Oncogene, Hepatocyte Growth Factor Receptor, AUTS9, Proto-Oncogene C-Met, HGFR, Scatter Factor Receptor, RCCP2, Tyrosine-Protein Kinase Met, c-Met, HGF Receptor, Met Proto-Oncogene Tyrosine Kinase, HGF/SF Receptor, SF Receptor, Hepatocyte growth factor receptor, SF receptor |
| Expressed Region | Glu25-Thr932 |
| Expression System | HEK293 cells |
| Tag | N-terminal 6x histidine tag |
| Purity | > 90% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | The recombinant human HGF receptor extracellular domain has a calculated molecular mass of 103 kDa. Due to the abundant glycosylation and cleavage, it migrates as an approximately 150, 80 and 45 kDa protein bands (confirmed by Western blotting) in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation under native and denature conditions, the protein presented as three reduced size bands. See deglycosylation analysis in SDS-PAGE image. |
| Format | Liquid |
| Formulation | Filtered solution in PBS |
| Concentration (lot specific) | Lot specific (see the label on the vial), determined by BCA protein assay kit (Thermo Scientific) |
| Preservative | None |
| Endotoxin Level | Not determined |
| Research Area | Cancer, Autophagy, Antibody Drug Development, Drug Antibody (DA) Detection, Protein Therapeutics, Receptor-Ligand Binding |
| Shipping Type | Blue ice |
| Storage | -20°C |
SDS-PAGE Image
Lane 1, protein standard ladder (kDa).
Lane 2, untreated protein.
Lane 3, treated protein with deglycosylation enzymes under native conditions.
Lane 4, treated protein with deglycosylation enzymes under denature conditions.
Lane 5, deglycosylation enzymes only without target proteins.
Activity
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.
Custom Labeling Request
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