Recombinant Human Trefoil Factor-3, expressed in E. coli
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Product Description
Specifications
| Size | 10 µg, 50 µg, 100 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | Q07654 |
| Gene Id | 7033 |
| Gene Symbols | TFF3 |
| Protein Name / Synonyms | Trefoil factor 3, TFF3. |
| Expressed Region | Glu22-Phe80 |
| Expression System | E. coli |
| Tag | N-terminal 6x histidine and thioredoxin tags |
| Purity | > 95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | 26.4 kDa |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS. Protein concentration prior to lyophilization was 0.78 mg/mL. |
| Preservative | None |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.1~ 1 mg/mL with sterile ice-cold deionized water or 1X❏, Phosphate Buffered Saline (PBS). This solution can then be further diluted into other aqueous buffers. |
| Endotoxin Level | Not determined |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
SDS-PAGE Image
Stability & Storage
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.
