Rabbit Anti-Trefoil factor 3(TFF3) Antibody
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Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | Q07654 |
| Gene Id | 7033 |
| Gene Symbols | TFF3 ITF TFI |
| Clonality | Polyclonal |
| Immunogen | The immunogen was a recombinant protein. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1: 8, 000), Western Blotting (recommended work dilution= 1:1000-5, 000) , (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein Inhibin alpha. The membrane blot was probed with anti- Inhibin alpha primary antibody (0.2 µg/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate recombinant protein Inhibin alpha from E.coli cell (14kDa). |
| Antigen Catalog Number | 230-00230 |
| Reconstitution | Product is supplied as a powder obtained from lyophilization of purified antibody in PBS without preservatives. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Trefoil factor 3 (TFF3) is a protein that has a trefoil motif characterized. Though those functions are not defined, they may protect the mucosa from insults. It can be used as a marker to improve the accuracy and acceptability of the detection/screening of Barrett's Oesophagus.
Specificity
This antibody binds to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. Cross reactivity with mouse and rat Trefoil factor 3 has not been tested yet.
Storage
Store at 4°C if intended for use within one month, otherwise, store at -20°C to -80°C. The lyophilized antibody is stable for at least 18 months after the date of receipt when stored at -20°C to -89°C. After reconstitution, it can be aliquoted and stored
Related Products
Mouse Anti-Human Trefoil factor 3 (Cat: DS-MB-03361) ; Trefoil factor 3, recombinant human protein, E.coli derived (Cat: 230-00228)
References
- Daniel K. Podolsky, et al. Colitis-associated variant of TLR2 causes impaired mucosal repair due to TFF3 deficiencyGastroenterology. 2009 July; 137(1): 209220.
- P. L. Beck, et al. Phylogenomic Exploring the interplay of barrier function and leukocyte recruitment in intestinal inflammation by targeting fucosyltransferase VII and trefoil factor 3. Am J Physiol Gastrointest Liver Physiol. 2010 July; 299(1): G43G53.
Expiration:
12 months from the date of shipment when stored properly.
