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Recombinant SARS-CoV-2 S1 Subunit Protein (RBD) with C-terminal Mouse IgG Fc Tag

Recombinant SARS-CoV-2 S1 subunit protein (RBD) with C-terminal mouse IgG Fc-tag, derived from the transfected human HEK293 cells.

Datasheet

$159.00  
230-30166-10
Datasheet
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Product Specifications

Size:
10 ug or 50 ug
Format:
Liquid
Formulation:
Supplied as a 0.2 um filtered solution in PBS (pH 7.4)
Purification:
Protein G affinity purification
Concentration:
Lot specific (see the label on the vial), determined by BCA protein assay
Purity:
>95 %
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Recommended Applications:
Binding assay, glycosylation analysis, biotin/dye/bead conjugation, other functional assays.

Source

Species:
SARS-CoV-2
Accession Number:
  • QHD43416
Expressed Region:
Arg319-Phe541
Protein Name & Synonyms:
Spike protein, S Protein, S1 Subunit, Host Cell Receptor Binding Domain (RBD)

Preparation

Expression System:
Human embryonic kidney 293 (HEK293) cells
Tag:
Fc
Conjugation/Tag Notes:
C-terminal mouse IgG Fc-tag
Molecular Weight (kDa):
Recombinant protein product has a calculated molecular mass of 50 kDa including 25 kDa mouse IgG Fc-tag. Due to the abundant glycosylation, it migrates as approximately ∼65 kDa major protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. See deglycosylation analysis image below.
SDS-PAGE Image
SDS-PAGE
Figure 1. Deglycosylation of purified recombinant proteins. Purified proteins were untreated (Lane 2) or treated with deglycosylation under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one major band at the expected size (∼50 kDa), thus indicating that the untreated recombinant protein (Lane 2, ∼65 kDa) was glycosylated.
Lane 1: Protein standard ladder (kDa)
Lane 2: Untreated protein (∼65 kDa, red arrow) under reducing conditions.
Lane 3: Treated protein (∼50 kDa, green arrow) with deglycosylation enzymes under native conditions.
Lane 4: Treated protein (∼50 kDa, green arrow) with deglycosylation enzymes under reducing conditions.
Binding Function
Binding Function
The human ACE2 protein product (Catalog #230-30165) was coated in 96-well plate and incubated with the serial diluted recombinant S1/RBD-Fc protein (Catalog #230-30166). The bound S1/RBD-Fc (Catalog #230-30166) was detected by HRP-conjugated anti-mouse IgG antibody using ELISA. The calculated EC50 is 9.85-13.46 ng/mL.

Stability & Storage

Shipping
Ice packs
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.

Related Coronavirus Proteins

Spike Protein, S1 Subunit
230-01101
Spike Protein, S2 Subunit
230-01103
Nucleocapsid Protein
230-01104
S1 Subunit Protein (RBD) with C-terminal Fc tag
230-20405
S1 Subunit Protein (RBD) with C-terminal His-tag
230-20406
S1 Subunit Protein with C-terminal His-tag
230-20407
S2 Subunit Protein with C-terminal His-tag
230-20408
Nucleocapsid Protein with C-terminal His-tag
230-20409

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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