Recombinant SARS-CoV-2 S1 Subunit Protein with C-terminal His-tag, Transfected HEK293 Cell Culture Supernatant

Recombinant SARS-CoV-2 S1 Subunit Protein with C-terminal His-tag, secreted from the serum-free medium of transfected HEK293 cells. Purchase will also include one vial of normal control (Catalog #. 230-20001), the culture supernatant of HEK293 cell transfected with empty expression vector.


Product Specifications

200 ul
Pink liquid
Cell culture supernatant of transfected HEK293 cells, serum-free.
Unpurified cell culture supernatant. HEK293 cells grown in serum-free medium were transfected with expression vector harboring target gene. The cell culture was harvested with centrifugation to remove cells. The cell culture supernatant containing mammalian cell protease inhibitor cocktail was aliquoted and stored at -80 °C immediately. The gene overexpression in culture supernatant was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the culture supernatant derived from HEK293 cells transfected with the empty expression vector was used as a negative control.


Accession Number:
  • QHD43416
Expressed Region:
Protein Name & Synonyms:
SARS-CoV-2 S1 Subunit Protein (RBD)


Expression System:
Human embryonic kidney 293 (HEK293) cells
Conjugation/Tag Notes:
C-terminal his-tag
Molecular Weight (kDa):
Recombinant protein product has a calculated molecular mass of 75. The actual molecular weight may increase slightly due to the potential post-translational modifications (PTMs).

Stability & Storage

Ice packs. Including one vial of cell culture supernatant of HEK293 cells transfected with empty expression vector.
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.

Related Coronavirus Proteins

Spike Protein, S1 Subunit
Spike Protein, S1 Subunit, Host Cell (RBD)
Spike Protein, S2 Subunit
Nucleocapsid Protein
S1 Subunit Protein (RBD) with C-terminal Fc tag
S1 Subunit Protein (RBD) with C-terminal His-tag
S2 Subunit Protein with C-terminal His-tag
Nucleocapsid Protein with C-terminal His-tag

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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.