Recombinant SARS-CoV-2 S1 Subunit Protein with C-terminal His-tag, Transfected HEK293 Cell Culture Supernatant

200 ul

Pink liquid

Cell culture supernatant of transfected HEK293 cells, serum-free.

Unpurified cell culture supernatant. HEK293 cells grown in serum-free medium were transfected with expression vector harboring target gene. The cell culture was harvested with centrifugation to remove cells. The cell culture supernatant containing mammalian cell protease inhibitor cocktail was aliquoted and stored at -80 °C immediately. The gene overexpression in culture supernatant was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the culture supernatant derived from HEK293 cells transfected with the empty expression vector was used as a negative control.

None

SARS-CoV-2

Val16-Gln690

SARS-CoV-2 S1 Subunit Protein (RBD)

Human embryonic kidney 293 (HEK293) cells

C-terminal his-tag

Recombinant protein product has a calculated molecular mass of 75. The actual molecular weight may increase slightly due to the potential post-translational modifications (PTMs).