Cell culture supernatant of transfected HEK293 cells, serum-free.
Purity:
Unpurified cell culture supernatant. HEK293 cells grown in serum-free medium were transfected with expression vector harboring target gene. The cell culture was harvested with centrifugation to remove cells. The cell culture supernatant containing mammalian cell protease inhibitor cocktail was aliquoted and stored at -80 °C immediately. The gene overexpression in culture supernatant was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the culture supernatant derived from HEK293 cells transfected with the empty expression vector was used as a negative control.
Preservative:
None
Source
Species:
SARS-CoV-2
Accession Number:
QHD43416
Expressed Region:
Val16-Gln690
Protein Name & Synonyms:
SARS-CoV-2 S1 Subunit Protein (RBD)
Preparation
Expression System:
Human embryonic kidney 293 (HEK293) cells
Conjugation/Tag Notes:
C-terminal his-tag
Molecular Weight (kDa):
Recombinant protein product has a calculated molecular mass of 75. The actual molecular weight may increase slightly due to the potential post-translational modifications (PTMs).
Stability & Storage
Shipping
Ice packs. Including one vial of cell culture supernatant of HEK293 cells transfected with empty expression vector.
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.