Supplied as a 0.2 um filtered solution in PBS (pH 7.4)
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Lot specific (see the label on the vial), determined by BCA protein assay
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Protein Name & Synonyms:
Spike protein, S Protein, S2 Subunit
Human embryonic kidney 293 (HEK293) cells
Molecular Weight (kDa):
Recombinant protein product has a calculated molecular mass of ∼60 kDa. Due to the abundant glycosylation, it migrates as approximately 80 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. See deglycosylation analysis image below.
Stability & Storage
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.