770-729-2992

Recombinant SARS-CoV-2 S2 Subunit Protein (full length)

Citations (1)

Recombinant SARS-CoV-2 S2 Subunit Protein with C-terminal tag, derived from the transfected human HEK293 cells.

Datasheet

$397.00  
230-30163-10
Datasheet
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Product Specifications

Size:
10 ug
Format:
Liquid
Formulation:
Supplied as a 0.2 um filtered solution in PBS (pH 7.4)
Purification:
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Concentration:
Lot specific (see the label on the vial), determined by BCA protein assay
Purity:
>90%
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining

Source

Species:
SARS-CoV-2
Accession Number:
  • QHD43416
Expressed Region:
Met697-Pro1213
Protein Name & Synonyms:
Spike protein, S Protein, S2 Subunit

Preparation

Expression System:
Human embryonic kidney 293 (HEK293) cells
Tag:
His
Conjugation/Tag Notes:
C-terminal his-tag
Molecular Weight (kDa):
Recombinant protein product has a calculated molecular mass of ∼60 kDa. Due to the abundant glycosylation, it migrates as approximately 80 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. See deglycosylation analysis image below.
SDS-PAGE Image
SDS-PAGE
Figure 1. Deglycosylation of purified recombinant proteins. Purified proteins were untreated (Lane 2) or treated with Protein Deglycosylation Kit under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one major band at the expected size (∼60 kDa), thus indicating that the untreated recombinant protein (Lane 2, ∼80 kDa) was glycosylated.
Lane 1: Protein standard ladder (kDa)
Lane 2: Untreated protein (∼80 kDa, red arrow) under reducing conditions
Lane 3: Partially cleaved protein (∼60 kDa, green arrow) with deglycosylation enzymes under native conditions
Lane 4: Fully cleaved protein (∼60 kDa, green arrow) with deglycosylation enzymes under reducing conditions

Stability & Storage

Shipping
Ice packs
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.

Related Coronavirus Proteins

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Spike Protein, S2 Subunit
230-01103
Nucleocapsid Protein
230-01104
S1 Subunit Protein (RBD) with C-terminal Fc tag
230-20405
S1 Subunit Protein (RBD) with C-terminal His-tag
230-20406
S1 Subunit Protein with C-terminal His-tag
230-20407
S2 Subunit Protein with C-terminal His-tag
230-20408
Nucleocapsid Protein with C-terminal His-tag
230-20409
  1. Shajahan A, et al. Deducing the N- and O- glycosylation profile of the spike protein of novel coronavirus SARS-CoV-2.?Glycobiology, cwaa042 (2020).
    Species:
    Sample type:

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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