Product Features
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves

Spiking & Recovery Results
| Sample Type | Average % Recovery | Range (%) |
|---|
| Serum | 107.0 | 97-113 |
| Plasma | 98.68 | 84-109 |
| Cell culture media | 128.8 | 125-132 |
Linearity Results
| Sample Type | Serum | Plasma | Cell Culture Media |
|---|
| 1:2 | Average % of Expected | 111.5 | 135.0 | 132.4 |
| Range (%) | 101-122 | 133-136 | 128-137 |
| 1:4 | Average % of Expected | 131.7 | 134.7 | 124.4 |
| Range (%) | 128-135 | 131-138 | 119-130 |
Kit Components
- Pre-Coated 96-well Strip Microplate
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- Wash Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
- Assay Diluent(s)
- Adhesive Sealing Film
Additional Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µL to 1 µL volumes
- Adjustable 1-25 µL pipettes for reagent preparation
- 100 µL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Log-log graph paper or computer and software for ELISA data analysis
- Tubes to prepare standard or sample dilutions
- Plastic wrap
Assay Procedure Summary
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µL standard or sample to each well. Incubate 2.5 hours at room temperature.
- Add 100 µL prepared biotin antibody to each well. Incubate 1 hour at room temperature.
- Add 100 µL prepared Streptavidin solution to each well. Incubate 45 minutes at room temperature.
- Add 100 µL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 µL Stop Solution to each well. Read at 450 nm immediately
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.