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NexaTag® Ultrasensitive Immunoassay Testing Services

Measuring low-abundance proteins from small or precious samples? Send them to our GLP-compliant laboratory. Our scientists run NexaTag® immuno-PCR assays on your samples and return quantitative results down to the fg/mL range.

Request a quote
Singleplex testing

One target per well

Multiplex testing

Up to 5 targets per well

100-Plex Human Profiling

100 targets from 150 µL

When to choose NexaTag® testing services

A good fit when…

  • Your targets are low-abundance and may fall below the range of a conventional ELISA
  • Sample volume is limited, such as small-animal, pediatric, or archived samples
  • You don’t have a qPCR instrument, or you’d rather not set up and validate the assays in-house.
  • You need results from a GLP-compliant laboratory to support a publication, grant, or collaboration.

Consider another option when…

  • You have a qPCR instrument and want to run the assays yourself.
    Browse NexaTag Kits
  • Your targets are abundant and sample is plentiful. A conventional ELISA may be all you need.
    Browse ELISA Kits
  • You’re screening hundreds to thousands of proteins for discovery. Our proteomics services cover far larger panels.
    Learn More

What we offer

Tell us your targets, sample type, and sample count, and we’ll recommend the format that fits.

Singleplex testing

We measure one target per well. A good choice when you’re following one or two key markers across a large sample set.

Multiplex testing

We measure up to 5 targets per well from the same small aliquot, so several related markers come from each sample without splitting it across assays.

NexaTag 100-Plex Human Profiling Service

Profile 100 human chemokines, growth factors, and inflammation markers from 150 µL of sample. Every target is reported as a concentration, not relative units.

100

Human chemokines, growth factors, and inflammation markers

150 µL

Sample required to profile all 100 targets

pg/mL - fg/mL

Limits of detection for each target

100-plex Profiling Panel

The NexaTag 100-Plex Human Profiling Service measures the 100 targets listed below, split into twenty 5-plex arrays: 8 chemokine arrays, 8 growth factor arrays, and 4 inflammation arrays. Run all twenty to profile every target, or choose only the arrays you need.

Why choose RayBiotech

Our years of experience

Founded in 2001 as the first company to commercialize the antibody array, we continue to use our decades of experience in multiplex, high-throughput protein analysis to deliver outstanding protein detection and analysis services to the research community.

GLP-compliant laboratory

Your samples are run in our GLP-compliant laboratory in Georgia, under documented procedures and quality controls at every step.

Small sample, broad coverage

NexaTag immuno-PCR detects proteins down to the fg/mL range, so a little sample goes a long way. That leaves more of your sample for repeat testing or other assays, which is especially important for small-animal, pediatric, and archived samples.

Our multiplexing capability

If you need multi-analyte detection, RayBiotech can multiplex up to 5 analytes per well with NexaTag immuno-PCR, all from a single small aliquot. In addition to our new 100-plex human profiling service, with twenty 5-plex arrays of chemokines, growth factors, and inflammation markers, we also provide panel customization to your specifications.

What you can expect

You

  1. Share your targets, sample type, and number of samples.
  2. Approve the quote and prepare your samples for shipment. You can find instructions here.
  3. Fill out a Service Form and include it with your shipment.
  4. Ship us your samples.

RayBiotech

  1. We will notify you once we receive your samples.
  2. We will run your samples in our GLP-compliant laboratory, keeping you informed about any issues or delays.
  3. We’ll send back a report with standard curves, Ct values, and sample concentrations for each target.
FAQ

Frequently Asked Questions

Still have questions?

Contact Us

Our NexaTag assays are validated for serum, plasma, and cell culture supernatants; however, our assays can generally accommodate many different sample types. Contact techsupport@raybiotech.com for questions about sample compatibility.

Singleplex testing and each 5-plex array require 20 µL of sample. The full 100-plex service needs 150 µL to cover all 100 targets.

The 100-plex profiling service is for human samples. For singleplex and multiplex testing, NexaTag assays detect targets from 16 species, including human, mouse, rat, dog, pig, and rhesus monkey. Contact us to confirm availability for your targets and species.

Standard curves, Ct values, and sample concentrations for each target.

Yes. We can multiplex up to 5 targets per well and customize panels to your specifications.

Yes. Concentrations are calculated from a standard curve and reported in pg/mL, not relative units.

Yes. Choose any number of the twenty 5-plex arrays. The more you bundle, the better the discount.

Still have questions?

Contact Us

Ready to send us your samples?

Tell us your targets, sample type, and sample count. Our experts will help you choose the right format and send a quote.

Request a quote
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Target

Array

LLOD (pg/mL)

UniProt

BLC

CHE1

0.39

O43927

Eotaxin

0.27

P51671

MIG

0.033

Q07325

Eotaxin-2

0.012

O00175

MIP-1 alpha

0.21

P10147

6Ckine

CHE2

0.13

O00585

Axl

0.5

P30530

Eotaxin-3

0.26

Q9Y258

HCC-4

4

O15467

Lymphotactin

48

P47992

CTACK

CHE3

1.5

Q9Y4X3

CXCL16

1

Q9H2A7

GCP-2

18

P80162

MCP-3

1.5

P80098

MIP-3 alpha

0.28

P78556

IP-10

CHE4

3

P02778

ENA-78

0.5

P42830

HCC-1

3

Q16627

I-TAC

0.5

O14625

MDC

0.43

O00626

IL-23

CHE5

3.3

Q9NPF7

CXCL14

50

O95715

MIF

0.56

P14174

MIP-3 beta

0.13

Q99731

TARC

0.8

Q92583

MIP-1 beta

CHE6

0.17

P13236

TSLP

0.17

Q969D9

RANK

26.8

Q9Y6Q6

IL-1 R5

2.4

Q13478

CXCL17

0.53

Q6UXB2

RANTES

CHE7

0.093

P13501

TIMP-1

0.9

P01033

PF-4

1

P02776

IGFBP-1

0.9

P08833

IGFBP-2

9

P18065

BMP-7

CHE8

2.9

P18075

CCL28

1.5

Q9NRJ3

TECK

0.4

O15444

IL-18 R beta

1.3

O95256

LIGHT

0.36

O43557

G-CSF

GF1

0.27

P09919

GM-CSF

0.33

P04141

FGF-17

2.4

O60258

FGF-21

0.26

Q9NSA1

M-CSF

0.9

P09603

FGF-7

GF2

0.01

P21781

bFGF

2.1

P09038

FGF-4

5

P08620

FGF-6

0.33

P10767

HB-EGF

0.002

Q99075

IL-28A

GF3

0.65

Q8IZJ0

BMP-4

10

P12644

BMP-9

0.51

Q9UK05

BTC

10

P35070

FGF-19

10

O95750

Activin A

GF4

2.1

P08476

IL-16

0.11

Q14005

AR

6

P15514

EG-VEGF

2.3

P58294

GDF-15

0.6

Q99988

PDGF-BB

GF5

0.007

P01127

IL-28B

1.17

Q8IZI9

SDF-1 alpha

5

P48061

NRG1-beta 1

14

Q02297

PDGF-AA

0.035

P04085

IL-21

GF6

2.8

Q9HBE4

FGF-9

4

P31371

GDNF

0.5

P39905

HGF

0.13

P14210

NT-4

0.5

P34130

IGFBP-4

GF7

45

P22692

TGF-alpha

0.02

P01135

VEGF-C

0.3

P49767

VEGF-D

1.5

O43915

Prolactin

10

P01236

OPN

GF8

6.4

P10451

EPO R

17.5

P19235

TGF-beta 2

15

P61812

GH

1

P01241

LIF

5

P15018

IFN-gamma

INF1

0.015

P01579

IL-12 p70

0.022

P29460

IL-1 beta

0.006

P01584

IL-6

0.03

P05231

TNF-alpha

0.025

P01375

IL-10

INF2

0.17

P22301

IL-13

0.015

P35225

IL-2

0.09

P60568

IL-4

0.45

P05112

IL-8

0.017

P10145

IL-1 alpha

INF3

0.38

P01583

IL-22

0.25

Q9GZX6

IL-5

0.78

P05113

IL-7

0.15

P13232

IL-12 p40

0.015

P29459

IL-1 F10

INF4

71

Q8WWZ1

Fas Ligand

0.2

P48023

IL-3

1.5

P08700

IL-24

34

Q13007

IL-33

2

O95760

Target

Array

LLOD (pg/mL)

UniProt

BLC

CHE1

0.39

O43927

Eotaxin

0.27

P51671

MIG

0.033

Q07325

Eotaxin-2

0.012

O00175

MIP-1 alpha

0.21

P10147

6Ckine

CHE2

0.13

O00585

Axl

0.5

P30530

Eotaxin-3

0.26

Q9Y258

HCC-4

4

O15467

Lymphotactin

48

P47992

CTACK

CHE3

1.5

Q9Y4X3

CXCL16

1

Q9H2A7

GCP-2

18

P80162

MCP-3

1.5

P80098

MIP-3 alpha

0.28

P78556

IP-10

CHE4

3

P02778

ENA-78

0.5

P42830

HCC-1

3

Q16627

I-TAC

0.5

O14625

MDC

0.43

O00626

IL-23

CHE5

3.3

Q9NPF7

CXCL14

50

O95715

MIF

0.56

P14174

MIP-3 beta

0.13

Q99731

TARC

0.8

Q92583

MIP-1 beta

CHE6

0.17

P13236

TSLP

0.17

Q969D9

RANK

26.8

Q9Y6Q6

IL-1 R5

2.4

Q13478

CXCL17

0.53

Q6UXB2

RANTES

CHE7

0.093

P13501

TIMP-1

0.9

P01033

PF-4

1

P02776

IGFBP-1

0.9

P08833

IGFBP-2

9

P18065

BMP-7

CHE8

2.9

P18075

CCL28

1.5

Q9NRJ3

TECK

0.4

O15444

IL-18 R beta

1.3

O95256

LIGHT

0.36

O43557

G-CSF

GF1

0.27

P09919

GM-CSF

0.33

P04141

FGF-17

2.4

O60258

FGF-21

0.26

Q9NSA1

M-CSF

0.9

P09603

FGF-7

GF2

0.01

P21781

bFGF

2.1

P09038

FGF-4

5

P08620

FGF-6

0.33

P10767

HB-EGF

0.002

Q99075

IL-28A

GF3

0.65

Q8IZJ0

BMP-4

10

P12644

BMP-9

0.51

Q9UK05

BTC

10

P35070

FGF-19

10

O95750

Activin A

GF4

2.1

P08476

IL-16

0.11

Q14005

AR

6

P15514

EG-VEGF

2.3

P58294

GDF-15

0.6

Q99988

PDGF-BB

GF5

0.007

P01127

IL-28B

1.17

Q8IZI9

SDF-1 alpha

5

P48061

NRG1-beta 1

14

Q02297

PDGF-AA

0.035

P04085

IL-21

GF6

2.8

Q9HBE4

FGF-9

4

P31371

GDNF

0.5

P39905

HGF

0.13

P14210

NT-4

0.5

P34130

IGFBP-4

GF7

45

P22692

TGF-alpha

0.02

P01135

VEGF-C

0.3

P49767

VEGF-D

1.5

O43915

Prolactin

10

P01236

OPN

GF8

6.4

P10451

EPO R

17.5

P19235

TGF-beta 2

15

P61812

GH

1

P01241

LIF

5

P15018

IFN-gamma

INF1

0.015

P01579

IL-12 p70

0.022

P29460

IL-1 beta

0.006

P01584

IL-6

0.03

P05231

TNF-alpha

0.025

P01375

IL-10

INF2

0.17

P22301

IL-13

0.015

P35225

IL-2

0.09

P60568

IL-4

0.45

P05112

IL-8

0.017

P10145

IL-1 alpha

INF3

0.38

P01583

IL-22

0.25

Q9GZX6

IL-5

0.78

P05113

IL-7

0.15

P13232

IL-12 p40

0.015

P29459

IL-1 F10

INF4

71

Q8WWZ1

Fas Ligand

0.2

P48023

IL-3

1.5

P08700

IL-24

34

Q13007

IL-33

2

O95760

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