Rat MIP-3 alpha/CCL20 ELISA Kit

RayBio® Rat MIP-3 alpha/CCL20 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Estimated Lead Time	1-2 business days
Species	Rat
Sensitivity	8.5 pg/ml Need more sensitivity? Check out the new NexaTag^® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa^™ technology.
Detection Range	8.5 pg/ml - 2000 pg/ml
Specificity	This ELISA kit shows no cross-reactivity with the following cytokines tested: Rat Activin A, beta-NGF, CINC-1, CINC-2, CINC-3, CNTF, Fractalkine, IL-1alpha, IL-1beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MMP-8, PDGF-AA, TIMP-1, TNF-alpha, VEGF.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum
Recommended Dilution (Serum/Plasma)	2 fold
Accession Number	P97884
Gene Id	29538
Gene Symbols	CCL20, SCYA20, ST38
Protein Name / Synonyms	C-C motif chemokine 20 (Beta-chemokine exodus-1) (CC chemokine LARC) (CC chemokine ST38) (Liver and activation-regulated chemokine) (Macrophage inflammatory protein 3 alpha) (MIP-3-alpha) (Small-inducible cytokine A20)
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Design Principle	Sandwich-based
Method Of Detection	Colorimetric
Research Area	Inflammation, Chemokine Biology
Shipping Type	Blue ice
Storage	≤-20°C

Product Features

Additional Notes

Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data

Application Notes

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Other Materials Required

Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation
100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Protocol Outline

Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Have this product in hand?

Get your lot-specific datasheet at Ray's Docs. The datasheet linked below is our current version and may not match your lot.

Datasheet (ELR-CCL20.pdf)

SDS (ELR-CCL20.pdf)

Excel (5af824478c7690acd2cf4518aeaae2058a44fa75.xlsm)

Product Features

Additional Notes

Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data

Application Notes

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Other Materials Required

Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation
100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Protocol Outline

Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Featured Products

Rat MIP-3 alpha/CCL20 ELISA Kit

Product Description

Specifications

Product Features

Application Notes

Storage/Stability

Have this product in hand?

Featured Products

Rat MIP-3 alpha/CCL20 ELISA Kit

Product Description

Specifications

NexaTag® Rat MIP-3 alpha (CCL20) qIPCR ELISA Kit

Product Features

Application Notes

Storage/Stability

Have this product in hand?

NexaTag® Rat MIP-3 alpha (CCL20) qIPCR ELISA Kit

Rat MIP-3 alpha/CCL20 ELISA Kit

Rat MIP-3 alpha/CCL20 ELISA Kit

Featured Products

Rat MIP-3 alpha/CCL20 ELISA Kit

Product Description

Specifications

Product Features

Application Notes

Storage/Stability

Documents

Have this product in hand?

Citations

Reviews

Ask A Question

Featured Products

Rat MIP-3 alpha/CCL20 ELISA Kit

Product Description

Specifications

Related Products

NexaTag® Rat MIP-3 alpha (CCL20) qIPCR ELISA Kit

Product Features

Application Notes

Storage/Stability

Documents

Have this product in hand?

Citations

Reviews

Ask A Question

Related Products

NexaTag® Rat MIP-3 alpha (CCL20) qIPCR ELISA Kit

Rat MIP-3 alpha/CCL20 ELISA Kit

Rat MIP-3 alpha/CCL20 ELISA Kit