The RayBio^® Acetylated-CD4 and Total-CD4 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human acetylated ATP-citrate synthase and total ATP-citrate synthase. An anti-pan CD4 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and CD4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-acetylation antibody is used to detect acetylated CD4 or biotinylated anti-CD4 antibody is used to detect total CD4 . After washing away unbound antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of acetylated CD4 or total CD4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

This assay can be developed for other species (mouse, rat, etc.) upon request. Contact techsupport@raybiotech.com for more information.