Introduction
Aldolase is a crucial enzyme in glycolysis and gluconeogenesis for energy production and glucose synthesis. It exists as three isoenzymes: Aldolase A (skeletal muscle and red blood cells; an indicator for late-stage muscular dystrophy, cancer, and myocardial infarction), Aldolase B (liver, kidney, and small intestine; associated with cirrhosis, hepatitis, and hereditary fructose intolerance), and Aldolase C (brain; associated with encephalitis, cerebral infarction, and some cancers). Monitoring aldolase levels is important in diagnostics and disease monitoring.
Assay Principle
Based on a coupled enzyme reaction, fructose-1,6-bisphosphate is catalyzed by aldolase to a colored product quantified at 450 nm. The change in absorbance is directly proportional to aldolase activity in the sample.
Product Features
- Simple, reproducible, and sensitive colorimetric assay in a 96-well microplate format
- Read on a standard microplate reader — no specialized instrumentation required
- Compatible with plasma, serum, cell lysates, and other biological fluids
- Includes a standard for quantification against a freshly prepared standard curve
- Results within a single workday
Standard Curve Range
NADH standard: 0–250 µM.
Additional Materials Required
- Microplate reader capable of measuring absorbance at the assay wavelength
- Precision and multi-channel pipettes
- Tubes for preparing sample dilutions
- MilliQ or double-distilled water (ddH2O)
Storage/Stability
Store the entire kit below -20°C for up to 6 months from the date of shipment. After preparation, store individual reagents as described in the kit manual and avoid repeated freeze-thaw cycles.
