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Anti-15-PGDH (C-term) Antibody

Catalog #: 107-10465

15-PGDH antibody [C2C3], C-Terminus, 1 mg/ml (100 ul)

Product Description

Specifications

Size100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman
Host SpeciesRabbit
Gene Id
3248
Protein Name / Synonyms
15-PGDH, C-term
Concentration (lot specific)
1 mg/ml
Recommended ApplicationsICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Research AreaNeuroscience
Shipping TypeBlue ice
Storage-20°C

Description

Synonyms

15-PGDH antibody, PGDH antibody, PGDH1 antibody, SDR36C1 antibody, HPGD antibody, prostaglandin dehydrogenase 1 antibody, NAD+-dependent 15-hydroxyprostaglandin dehydrogenase antibody, 15-hydroxyprostaglandin dehydrogenase [NAD+] antibody, "short chain dehydrogenase/reductase family 36C, member 1 antibody", hydroxyprostaglandin dehydrogenase 15-(NAD) antibody

Specificity

Species Reactivity
Human

Format

Product Type
Primary antibody
Formulation
1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
29 kDa

Immunogen

Synthetic peptide corresponding to a region within amino acids 205 and 266 of 15-PGDH (Uniprot ID# P15428)

Applications

Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 15-PGDH(107-10465) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft, using 15-PGDH(107-10465) antibody at 1:500 dilution.
Sample (30µg whole cell lysate)
A:Raji
12% SDS PAGE
107-10465 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.