Anti-27Kip1 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P46527
Gene Id	1027
Gene Symbols	CDKN1B
Protein Name / Synonyms	27Kip1 (C-term)
Clonality	Polyclonal
Preparation	This 27Kip1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 165-194 amino acids from the C-terminal region of human 27Kip1.
Concentration (lot specific)	0.420 mg/ml
Recommended Applications	Western Blotting, IF
Research Area	Cardiovascular Disease, Cancer, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

p27Kip1 is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

Specificity

Predicted Reactivity

Hamster

Other Names

CDKN1B; KIP1; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; p27Kip1

NCBI Accession #

NP_004055.1

Other Accession #

Q60439

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

22073

Recommended Dilutions

WB: 1:1000; IF: 1:10-50

	Western blot analysis of p27Kip1 Antibody (C-term)(Cat#6269b) in HepG2 cell line lysates (35ug/lane). 27Kip1(arrow) was detected using the purified Pab.
	Confocal immunofluorescent analysis of 27Kip1 Antibody (C-term)(Cat# 102-16711) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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