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Anti-A-RAF Antibody
Catalog #: 107-10259
A-RAF antibody [C1C3], 0.85 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 369 |
| Protein Name / Synonyms | A-RAF |
| Concentration (lot specific) | 0.85 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Research Area | Ras/Rap Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
A-RAF antibody, ARAF1 antibody, PKS2 antibody, RAFA1 antibody, ARAF antibody, proto-oncogene Pks antibody, serine/threonine-protein kinase A-Raf antibody, A-Raf proto-oncogene serine/threonine-protein kinase antibody, v-raf murine sarcoma 3611 viral oncogene homolog 1 antibody, Oncogene ARAF1 antibody, Ras-binding protein DA-Raf antibody, proto-oncogene A-Raf-1 antibody, proto-oncogene A-Raf antibody, v-raf murine sarcoma 3611 viral oncogene homolog antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
68 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 371 and 554 of A-RAF (Uniprot ID# P10398)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using ARAF(107-10259) antibody at 1:500 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed A431, using ARAF(107-10259) antibody at 1:200 dilution.
Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
107-10259 diluted at 1:1000
A: Hela
7.5% SDS PAGE
107-10259 diluted at 1:1000
Sample (30 ug of whole cell lysate)
A:NIH-3T3
7.5% SDS PAGE
107-10259 diluted at 1:1000
A:NIH-3T3
7.5% SDS PAGE
107-10259 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.