Anti-ABI1 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q8IZP0
Gene Id	10006
Gene Symbols	ABI1
Protein Name / Synonyms	ABI1 (N-term)
Clonality	Polyclonal
Preparation	This ABI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-108 amino acids from the N-terminal region of human ABI1.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

ABI1 has been found to form a complex with EPS8 and SOS1, and is thought to be involved in the transduction of signals from Ras to Rac. In addition, this protein may play a role in the regulation of EGF-induced Erk pathway activation as well as cytoskeletal reorganization and EGFR signaling.

Specificity

Predicted Reactivity

Mouse, Rat

Other Names

ABI1; SSH3BP1; Abl interactor 1; Abelson interactor 1; Abl-binding protein 4; Eps8 SH3 domain-binding protein; Nap1-binding protein; Spectrin SH3 domain-binding protein 1; e3B1

NCBI Accession #

NP_001012768.1;NP_001012769.1;NP_001012770.1;NP_001171587.1;NP_001171590.1;NP_001171591.1;NP_001171592.1;NP_001171593.1;NP_001171594.1;NP_001171595.1;NP_001171596.1;NP_005461.2

Other Accession #

P62484; Q9NYB9; Q9QZM5; Q8CBW3

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

55081

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50; IF: 1:10-50

	Western blot analysis of ABI1 Antibody (N-term) (Cat# 102-17906) in MCF-7, CEM, Jurkat cell line lysates (35ug/lane). ABI1 (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human brain tissue reacted with ABI1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Flow cytometric analysis of SK-Br-3 cells using ABI1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Immunofluorescence analysis of ABI1 Antibody (N-term) with paraffin-embedded human brain tissue . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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