AChE antibody, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 43 |
| Protein Name / Synonyms | AChE |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
ARACHE antibody, N-ACHE antibody, YT antibody, ACHE antibody, Yt blood group antibody, acetylcholinesterase antibody, apoptosis-related acetylcholinesterase antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
68 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 406 and 614 of AChE (Uniprot ID# P22303)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using AChE(107-10274) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2500.
AChE antibody detects ACHE protein at cytoplasm on colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
AChE antibody (107-10274) dilution: 1:500.
Sample: Paraffin-embedded colon carcinoma.
AChE antibody (107-10274) dilution: 1:500.
AChE antibody detects AChE protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
AChE antibody (107-10274) dilution: 1:500.
Sample: Paraffin-embedded mouse colon.
AChE antibody (107-10274) dilution: 1:500.
AChE antibody detects AChE protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
AChE antibody (107-10274) dilution: 1:500.
Sample: Paraffin-embedded rat fore brain.
AChE antibody (107-10274) dilution: 1:500.
Sample (30 ug of whole cell lysate)
A: Raji
7.5% SDS PAGE
107-10274 diluted at 1:1000
A: Raji
7.5% SDS PAGE
107-10274 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.
