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Anti-ACLY Antibody
Catalog #: 144-61516
Phospho-ACLY-S455 pAb
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P53396 |
| Gene Id | 47 |
| Gene Symbols | ACLY |
| Protein Name / Synonyms | ACLY, ACL, ATPCL, CLATP, ATP citrate lyase |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IP 1:50 - 1:100
IHC 1:50 - 1:100
IP 1:50 - 1:100
Format
Calculated MW
91kDa/119kDa/120kDa
Observed MW
121kDa
Immunogen
A synthetic phosphorylated peptide around S455 of human ACLY (NP_001087.2).
Modification
Phospho S455
Storage
-20°C
Images
Western blot analysis of extracts of NIH/3T3 cells,using Phospho-ACLY-S455 antibody (144-61516) at 1:2000 dilution. NIH/3T3 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat pancreas using Phospho-ACLY-S455 antibody (144-61516) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.