Anti-ACTG1 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P63261
Gene Id	71
Gene Symbols	ACTG1
Protein Name / Synonyms	ACTG1 (Center)
Clonality	Polyclonal
Preparation	This ACTG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188-215 amino acids from the Central region of human ACTG1.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, FC, IF
Research Area	Angiogenesis
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, is a cytoplasmic actin found in nonmuscle cells.

Specificity

Predicted Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Drosophila, C.Elegans, Hamster, Pig, Monkey, Rabbit, Xenopus, Yeast

Other Names

ACTG1; ACTB; ACTG; Actin, cytoplasmic 2; Gamma-actin; Actin, cytoplasmic 2, N-terminally processed

NCBI Accession #

NP_001186883.1;NP_001605.1

Other Accession #

P60010; P68136; P68135; P68137; P68134; P68133; P68139; P68138; P63269; P63268; P63267; P63270; Q5E9B5; A2BDB0; P63259; P63260; Q5ZMQ2; P63258; P04751; P68035; P68033; P68032; P68034; Q3ZC07; O93400; P60711; P29751; Q6QAQ1; P60710; Q4R561; P60709; P48975;

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

41793

Recommended Dilutions

WB: 1:1000; FC: 1:10-50; IF: 1:10-50

	Western blot analysis of ACTG1 Antibody (Center) (Cat# 102-16809) in K562, HepG2 cell line lysates (35ug/lane). ACTG1 (arrow) was detected using the purified Pab.
	Flow cytometric analysis of K562 cells using ACTG1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Western blot analysis of ACTG1 (arrow) using rabbit polyclonal ACTG1 Antibody (Center) (Cat# 102-16809). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ACTG1 gene.
	Confocal immunofluorescent analysis of ACTG1 Antibody (Center)(Cat# 102-16809) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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