Anti-Actin (ACTB/ACTC) (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P68032
Gene Id	70
Gene Symbols	ACTC1
Protein Name / Synonyms	Actin (ACTB/ACTC) (Center)
Clonality	Polyclonal
Preparation	This Actin (ACTB/ACTC) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 189-218 amino acids from the Central region of human Actin (ACTB/ACTC).
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Cardiovascular Disease, Cytoskeletal Reorganization
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. ACTB/ACTC are nonmuscle cytoskeletal actins and major constituents of the contractile apparatus. Defects in ACTB are a cause of juvenile-onset dystonia. Defects in ACTC have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC).

Specificity

Predicted Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Drosophila, C.Elegans, Hamster, Pig, Monkey, Rabbit, Xenopus, Yeast

Other Names

ACTC1; ACTC; Actin, alpha cardiac muscle 1; Alpha-cardiac actin

NCBI Accession #

NP_005150.1

Other Accession #

P60010; P68136; P68135; P68137; P68134; P68133; P68139; P68138; P63269; P63268; P63267; P63270; Q5E9B5; A2BDB0; P63259; P63260; P63261; Q5ZMQ2; P63258; P04751; P68035; P68033; P68034; Q3ZC07; O93400; P60711; P29751; Q6QAQ1; P60710; Q4R561; P60709; P48975;

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

42019

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50

	Western blot analysis of ACTB/ACTC Antibody (Center) in HL60 cell line lysates (35ug/lane). Target protein (arrow) was detected using the purified Pab.
	Western blot analysis of ACTB/ACTC (arrow) using rabbit polyclonal ACTB/ACTC Antibody (Center) (Cat# 102-12723).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACTB/ACTC gene (Lane 2) (Origene Technologies).
	Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with ACTB/ACTC antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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