Anti-Actin (ACTB/ACTC) (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P68032
Gene Id	70
Gene Symbols	ACTC1
Protein Name / Synonyms	Actin (ACTB/ACTC) (N-term)
Clonality	Polyclonal
Preparation	This Actin (ACTB/ACTC) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38-67 amino acids from the N-terminal region of human Actin (ACTB/ACTC).
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Research Area	Cardiovascular Disease, Cytoskeletal Reorganization
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. ACTB/ACTC are nonmuscle cytoskeletal actins and major constituents of the contractile apparatus. Defects in ACTB are a cause of juvenile-onset dystonia. Defects in ACTC have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC).

Specificity

Predicted Reactivity

Rat, Zebrafish, Bovine, Chicken, Drosophila, C.Elegans, Hamster, Pig, Monkey, Rabbit, Xenopus, Yeast

Other Names

ACTC1; ACTC; Actin, alpha cardiac muscle 1; Alpha-cardiac actin

NCBI Accession #

NP_005150.1

Other Accession #

P60010; P68136; P68135; P68137; P68134; P68133; P68139; P68138; P63269; P63268; P63267; P63270; Q5E9B5; A2BDB0; P63259; P63260; P63261; Q5ZMQ2; P63258; P04751; P68035; P68033; P68034; Q3ZC07; O93400; P60711; P29751; Q6QAQ1; P60710; Q4R561; P60709; P48975;

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

42019

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; IF: 1:10-50

	Western blot analysis of ACTB/ACTC Antibody (N-term) in mouse testis tissue lysates (35ug/lane). Target protein (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ACTB/ACTC antibody (N-term) (Cat# 102-12723), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Confocal immunofluorescent analysis of Actin (ACTB/ACTC) Antibody (N-term)(Cat# 102-12723) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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