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Anti-Actin (SMA) Antibody
Catalog #: 130-10038
Rabbit Anti-Human Actin (SMA) Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P63236 |
| Gene Id | 1024915, 93775651 |
| Gene Symbols | gadC acsA xasA SF1735 S1868 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was synthetic peptide derived from human Smooth Muscle Actin. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation, and followed by Protein A/G affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1: 80, 000(at least detecting 12.5ng/ml)) |
| Reconstitution | Supplied as lyophilized and purified antibody originally containing PBS, without Preservative Stabilizers, such as Sodium Azide. The final concentration is indicated on the shipping vial. The antibody is stable for at least years from the data of receipt when stored at -20°C to -70°C. Reconstituted antibody (recommended with H20 1 mg/ml) can also be aliquoted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss activity. Upon reconstitution, the antibody can also be stored over months at 4°C. Please avoid freeze-thaw cycles. |
| Research Area | Cytoskeletal Reorganization |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Actin is a globular, roughly 42-kDa protein found in all eukaryotic cells (the only known exception being nematode sperm). It is also one of the most highly-conserved proteins, differing by no more than 20% in species as diverse as algae and humans. Actin is the monomeric subunit of two types of filaments in cells: microfilaments, one of the three major components of the cytoskeleton, and thin filaments, part of the contractile apparatus in muscle cells. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. Further more, expression of smooth muscle alpha actin is regulated by hormones, cell proliferation , and altered by pathological conditions including oncogenic transformation and atherosclerosis.
Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. Further more, expression of smooth muscle alpha actin is regulated by hormones, cell proliferation , and altered by pathological conditions including oncogenic transformation and atherosclerosis.
Specificity
The antibody can specifically bind to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. The specificity for binding to recombinant protein, cellular protein and native antigen is not defined. Cross reactivity with mouse and rat Smooth Muscle has not yet been tested.
Storage
Keep it at 4°C if intended for use within a month. Store at -20°C if over months. Minimize freezing and thawing when use.
Related Products
Mouse Anti-Smooth Muscle Actin (cat# MD-14-0004 ) Mouse Anti-Smooth Muscle Actin (cat# MD-14-0003) Goat Anti-Actin (Smooth Muscle), ( cat# ER-14-0084)
References
- Sjöblom B, et al.. Alpha-actin in structure and regulation. Cell Mol Life Sci. 2008 Sep;65(17):2688-701.
- Milewicz D.M.,ET.AL,, De novo ACTA2 mutation causes a novel syndrome of multisystemic smooth muscle dysfunction. Am. J. Med. Genet. A 152:2437-2443(2010) [PubMed: 20734336
Expiration:
12 months from the date of shipment when stored properly.