Anti-ACVRL1 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P37023
Gene Id	94
Gene Symbols	ACVRL1
Protein Name / Synonyms	ACVRL1 (N-term)
Clonality	Polyclonal
Preparation	This ACVRL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38-68 amino acids from the N-terminal region of human ACVRL1.
Concentration (lot specific)	2.000 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Research Area	Angiogenesis, Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

ACVRL1 is a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. This protein, sometimes termed ALK1, shares similar domain structures with other closely related ALK or activin receptor-like kinase proteins that form a subfamily of receptor serine/threonine kinases. Mutations in this gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2.

Specificity

Other Names

ACVRL1; ACVRLK1; ALK1; Serine/threonine-protein kinase receptor R3; Activin receptor-like kinase 1; TGF-B superfamily receptor type I

NCBI Accession #

NP_000011.2;NP_001070869.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

56124

Recommended Dilutions

WB: 1:1000; FC: 1:10-50; IHC: 1:50-100

	Western blot of ACVRL1 Pab (Cat# 102-17633). TOP LEFT: Mouse heart tissue lysate.
	Western blot analysis of ACVRL1 (arrow) using rabbit polyclonal ACVRL1 Antibody (N-term) (Cat# 102-17633). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACVRL1 gene (Lane 2) (Origene Technologies).
	Flow cytometric analysis of HepG2 cells using ACVRL1 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
	human chondrocytes (C28/I2 cells), transfected with empty vector (lane 1, 3) or ACVRL1(lane 2, 4). RIPA lysis buffer, 20 ug/lane of protein, primary antibody dilution 1:1000, blocking solution is 5% milk in TBST (lane 1 and 2), 5% BSA in TBST (lane 3 and 4). Data courtesy of Kenneth Finnson, Montreal General Hospital.