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Anti-ADAR1 (internal) Antibody
Catalog #: 107-10272
ADAR1 antibody [N3C1], Internal, 0.54 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 103 |
| Protein Name / Synonyms | ADAR1, Internal |
| Concentration (lot specific) | 0.54 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
ADAR1 antibody, DRADA antibody, DSH antibody, DSRAD antibody, G1P1 antibody, IFI-4 antibody, IFI4 antibody, K88DSRBP antibody, P136 antibody, ADAR antibody, 136 kDa double-stranded RNA-binding protein antibody, dsRNA adenosine deaminase antibody, interferon-inducible protein 4 antibody, interferon-induced protein 4 antibody, double-stranded RNA-specific adenosine deaminase antibody, adenosine deaminase acting on RNA 1-A antibody, "adenosine deaminase, RNA-specific antibody"
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
136 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 472 and 757 of ADAR1 (Uniprot ID# P55265)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded Colon ca, using ADAR1(107-10272) antibody at 1:250 dilution.
Sample (30 ug of whole cell lysate)
A: 293T
5% SDS PAGE
107-10272 diluted at 1:1000
A: 293T
5% SDS PAGE
107-10272 diluted at 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ADAR1(107-10272) antibody at 1:200 dilution.
Sample (30 ug of whole cell lysate)
A:NIH-3T3 5% SDS PAGE
107-10272 diluted at 1:1000
A:NIH-3T3 5% SDS PAGE
107-10272 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.