Anti-ADH1C (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P00326
Gene Id	126
Gene Symbols	ADH1C
Protein Name / Synonyms	ADH1C (Center)
Clonality	Polyclonal
Preparation	This ADH1C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 231-260 amino acids from the Central region of human ADH1C.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

This gene encodes class I alcohol dehydrogenase, gamma subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class I alcohol dehydrogenase, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster.

Specificity

Other Names

ADH1C; ADH3; Alcohol dehydrogenase 1C; Alcohol dehydrogenase subunit gamma

NCBI Accession #

NP_000660.1

Other Accession #

P00325; P07327; NP_000660.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

39868

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; IF: 1:10-50; FC: 1:10-50

	ADH1C Antibody (Center) (Cat# 102-16525) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the ADH1C antibody detected the ADH1C protein (arrow).
	Western blot analysis of ADH1C (arrow) using rabbit polyclonal ADH1C Antibody (Center) (Cat# 102-16525). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ADH1C gene.
	ADH1C Antibody (Center) (Cat# 102-16525) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ADH1C Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
	Confocal immunofluorescent analysis of ADH1C Antibody (Center)(Cat# 102-16525) with T47D cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
	ADH1C Antibody (Center) (Cat# 102-16525) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.