Anti-Adiponectin/Acrp30 Antibody, Biotinylated

Adiponectin is composed of four distinct domains: a signal peptide at the N terminus, a short variable region, a collagenous domain, and a C-terminal globular domain homologous to C1q. The crystal structure of adiponectin globular domain reveals a striking resemblance to the structure of tumor necrosis factor (TNF)-alpha. Adiponectin belongs to a growing family of proteins, all of which contain a Cterminal globular C1q-like domain of ?135 aa. Most of them also contain a variable number of “Gly-XY” (where X and Y represent any amino acid) collagenous repeats. Adiponectin is expressed exclusively by differentiated adipocytes and its expression is induced ?100-fold during adipocyte differentiation. Serum levels of adiponectin correlate inversely with insulin sensitivity. In healthy adults, adiponectin circulates in serum at a high concentration (1.9–17.0 µg/ml). Adiponectin levels are significantly reduced in a variety of obese and insulin-resistant states in mice, monkeys, and humans.

The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.

The rabbit anti-human Acrp30 antibody detected specifically human recombinant protein and cellular protein from cell lysates at various concentrations. Cross reactivity with mouse and rat are expected from sequence similarity.