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Anti-Adiponectin (ADIPOQ) Antibody, Biotinylated
Catalog #: 130-10145B-50
Rabbit anti-Adiponectin (ADIPOQ) Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | AAH96308 |
| Clonality | Polyclonal |
| Immunogen | The immunogen was a recombinant protein. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography. |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1: 8, 000), Western Blotting (recommended work dilution= 1:1000-5, 000) , (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1.recombinant protein ADIPOQ.The membrane blot was probed with anti-ADIPOQ primary antibody (0.2 µg/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate recombinant proteinADIPOQ from E.coli cell (15kDa). |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Research Area | Cardiovascular Disease, Energy Metabolism, Obesity & Metabolic Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Human Adiponectin (ADIPOQ) is a protein involved in the control of fat metabolism and insulin sensitivity. Adiponectin/APM1 is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. APM1 gene is exclusively expressed in adipose tissue. The protein, also known as gelatin-binding protein, has high similarity with collagens X and VIII and complement factor C1q. Studies suggest that APM1 protein may be a collagen-like secretory protein. Defects in adiponectin are the cause of adiponectin deficiency. The result is a very low concentration of plasma adiponectin which is associated with obesity insulin resistance, and diabetes type 2.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
This antibody binds to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. Cross reactivity with mouse and rat ADIPOQ has not been tested yet.
Related Products
RayBio® Human Adiponectin / Acrp30 ELISA Kit For Serum, Plasma, Cell Culture Supernatant and Urine (Cat: ELH-Adiponectin-001) RayBio® Adiponectin / Acrp30 EIA Kit for Serum, Plasma, Culture Supernatant and Cell Lysates (QAH-CUST) Mouse Anti-Adiponectin / Acrp30 (Cat: MD-14-0734)
References
- Katherine Robinson,et al. Clinical review: Adiponectin biology and its role in inflammation and critical illness. Crit Care. 2011; 15(2): 221.
- Dominik Kraus,et al. Interactions of Adiponectin and Lipopolysaccharide from Porphyromonas gingivalis on Human Oral Epithelial Cells. PLoS One. 2012; 7(2): e30716.
Expiration:
12 months from the date of shipment when stored properly.