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Anti-Adipsin/Factor D Antibody
Catalog #: RB-08-0017
Rabbit Anti-Human Adipsin/Factor D Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | NM_001928 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was synthetic peptide derived from Human Adipsin. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation followed by Protein A/G affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1: 5, 000-10, 000), Western Blotting (recommended work dilution= 1:1000-2000) |
| Reconstitution | The purified antibody is supplied lyophilized originally containing PBS without any preservative stabilizers. The lyophilized antibody is stable for at least a year from the date of receipt when stored frozen at -20°C to -70°C. It is suggested that the antibody is reconstituted with sterile water and can be aliquoted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss of activity. Upon reconstitution, the antibody can also be stored for months at 4°C. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Adipsin is also called as complement factor D, or C3 convertase activator or properdin factor D, because they are identical molecule, mature protein aa 26-253, ~22kD. It is belonged to a serine protease that is secreted by adipocytes. Adipocytes synthesize and store energy in periods of nutritional abundance and mobilize lipids during starvation and other times of need. The switch from energy storage to expenditure is finely regulated by a variety of hormones. In order to accomplish these complex tasks energy balance, adipocytes express many genes, including adipsin, involved in lipid metabolism and glucose homeostasis. Many of these genes are finely regulated during adipocyte differentiation and maturation. Several adipocyte-derived proteins act in an autocrine or paracrine fashion to control its own and other cell's cellular physiology.
Specificity
The rabbit anti-human Adipsin antibody detects targets derived from human recombinant and cellular proteins at various concentrations. Cross reactivity with mouse and rat are expected from sequence similarity.
Storage
Store at -20°C, if not intended for use within a month, keep it at 4°C and minimize freezing and thawing when use.
References
- White R.T., Damm D., and et.al. Human adipsin is identical to complement factor D and is expressed at high levels in adipose tissue. J. Biol. Chem. 267:9210-9213(1992)
- Davis A.E. III, Active site amino acid sequence of human factor D. Proc. Natl. Acad. Sci. U.S.A. 77:4938-4942(1980)
Expiration:
12 months from the date of shipment when stored properly.